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Crystal structure of catalytically inactive FnCas12a in complex with a crRNA guide and a dsDNA target

X-ray diffraction
2.65Å resolution
PDB entry 6i1k coloured by chain, front view.
PDB entry 6i1k coloured by chain, side view.
PDB entry 6i1k coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CRISPR-associated endonuclease Cas12a and RNA and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 73833.5 Å2
Buried surface area: 22779.39 Å2
Dissociation area: 2,612.5 Å2
Dissociation energy (ΔGdiss): 45.24 kcal/mol
Dissociation entropy (TΔSdiss): 13.94 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106194

Macromolecules

CRISPR-associated endonuclease Cas12a
Chain: A
Length: 1302 amino acids
Theoretical weight: 152.28 KDa
Source organism: Francisella tularensis subsp. novicida U112
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0Q7Q2 (Residues: 2-1300; Coverage: 100%)
Gene names: FTN_1397, cas12a, cpf1
Pfam:
InterPro:
CRISPR-associated endonuclease Cas12a in PDB entry 6i1k, assembly 1, front view.
CRISPR-associated endonuclease Cas12a in PDB entry 6i1k, assembly 1, side view.
CRISPR-associated endonuclease Cas12a in PDB entry 6i1k, assembly 1, top view.
PDBe-KB: UniProt Coverage View: A0Q7Q2  
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DNA chain C
Name: DNA target strand (38-MER)
Representative chains: C
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 38 nucleotides
Theoretical weight: 11.67 KDa
DNA target strand (38-MER) in PDB entry 6i1k, assembly 1, front view.
DNA target strand (38-MER) in PDB entry 6i1k, assembly 1, side view.
DNA target strand (38-MER) in PDB entry 6i1k, assembly 1, top view.
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DNA chain D
Name: DNA non-target strand (33-MER)
Representative chains: D
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 38 nucleotides
Theoretical weight: 11.62 KDa
DNA non-target strand (33-MER) in PDB entry 6i1k, assembly 1, front view.
DNA non-target strand (33-MER) in PDB entry 6i1k, assembly 1, side view.
DNA non-target strand (33-MER) in PDB entry 6i1k, assembly 1, top view.
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RNA chain B
Name: crRNA (40-MER)
Representative chains: B
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 40 nucleotides
Theoretical weight: 12.75 KDa
crRNA (40-MER) in PDB entry 6i1k, assembly 1, front view.
crRNA (40-MER) in PDB entry 6i1k, assembly 1, side view.
crRNA (40-MER) in PDB entry 6i1k, assembly 1, top view.
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