› 6ien

X-ray diffraction
2.7Å resolution

Substrate/product bound Argininosuccinate lyase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb6ien/pdb
PDB entry 6ien coloured by chain, front view.
PDB entry 6ien coloured by chain, side view.
PDB entry 6ien coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural studies on M. tuberculosis argininosuccinate lyase and its liganded complex: Insights into catalytic mechanism.
Paul A, Mishra A, Surolia A, Vijayan M
IUBMB Life 71 643-652 (2019)
PMID: 30615268

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 470 amino acids
Theoretical weight: 49.83 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPY7 (Residues: 1-470; Coverage: 100%)
Gene names: MTCY06H11.24, Rv1659, argH
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand AS1 3 x AS1
Ligand FUM 1 x FUM
Ligand EDO 5 x EDO
Ligand PEG 1 x PEG
Ligand ARG 1 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 114.18Å b: 131.35Å c: 145.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.246 0.293
Expression system: Escherichia coli

Quick links