Structure analysis

Cryo-EM structure of the CMV-stalled human 80S ribosome with HCV IRES (Structure iii)

Electron Microscopy
4.5Å resolution
Source organism: Homo sapiens
Assembly composition:
hetero 83-mer (preferred)
Entry contents: 76 distinct polypeptide molecules
7 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 83-mer
PDBe Complex ID: PDB-CPX-140324

Macromolecules

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Chain: 1G
Length: 297 amino acids
Theoretical weight: 34.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46777 (Residues: 1-297; Coverage: 100%)
Gene names: MSTP030, RPL5
Pfam:
InterPro:

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Chain: 2F
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P26373 (Residues: 1-211; Coverage: 100%)
Gene names: BBC1, OK/SW-cl.46, RPL13
Pfam: Ribosomal protein L13e
InterPro:

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Chain: 2H
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61313 (Residues: 1-204; Coverage: 100%)
Gene names: EC45, RPL15, TCBAP0781
Pfam: Ribosomal L15
InterPro:

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Chain: 2I
Length: 203 amino acids
Theoretical weight: 23.63 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P40429 (Residues: 1-203; Coverage: 100%)
Gene name: RPL13A
Pfam: Ribosomal protein L13
InterPro:

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Chain: 2J
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18621 (Residues: 1-184; Coverage: 100%)
Gene name: RPL17
Pfam: Ribosomal protein L22p/L17e
InterPro:

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Chain: 2K
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q07020 (Residues: 1-188; Coverage: 100%)
Gene name: RPL18
Pfam: Ribosomal protein 60S L18 and 50S L18e
InterPro:

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Chain: 2M
Length: 176 amino acids
Theoretical weight: 20.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02543 (Residues: 1-176; Coverage: 100%)
Gene name: RPL18A
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
InterPro:

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Chain: 2N
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46778 (Residues: 1-160; Coverage: 100%)
Gene name: RPL21
Pfam: Ribosomal protein L21e
InterPro:

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Chain: 2O
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P35268 (Residues: 1-128; Coverage: 100%)
Gene name: RPL22
Pfam: Ribosomal L22e protein family
InterPro:

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Chain: 2P
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62829 (Residues: 1-140; Coverage: 100%)
Gene name: RPL23
Pfam: Ribosomal protein L14p/L23e
InterPro:

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Chain: 2Q
Length: 157 amino acids
Theoretical weight: 17.83 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P83731 (Residues: 1-157; Coverage: 100%)
Gene name: RPL24
Pfam: Ribosomal protein L24e
InterPro:

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Chain: 2V
Length: 159 amino acids
Theoretical weight: 17.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P47914 (Residues: 1-159; Coverage: 100%)
Gene name: RPL29
Pfam: Ribosomal L29e protein family
InterPro: Large ribosomal subunit protein eL29

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Chain: 2X
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62899 (Residues: 1-125; Coverage: 100%)
Gene name: RPL31
Pfam: Ribosomal protein L31e
InterPro:

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Chain: 2Y
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62910 (Residues: 1-135; Coverage: 100%)
Gene names: PP9932, RPL32
Pfam: Ribosomal protein L32
InterPro:

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Chain: 2Z
Length: 110 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18077 (Residues: 1-110; Coverage: 100%)
Gene names: GIG33, RPL35A
Pfam: Ribosomal protein L35Ae
InterPro:

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Chain: 2a
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49207 (Residues: 1-117; Coverage: 100%)
Gene name: RPL34
Pfam: Ribosomal protein L34e
InterPro:

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Chain: 2b
Length: 123 amino acids
Theoretical weight: 14.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P42766 (Residues: 1-123; Coverage: 100%)
Gene name: RPL35
Pfam: Ribosomal L29 protein
InterPro:

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Chain: 2c
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y3U8 (Residues: 1-105; Coverage: 100%)
Gene name: RPL36
Pfam: Ribosomal protein L36e
InterPro:

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Chain: 2d
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61927 (Residues: 1-97; Coverage: 100%)
Gene name: RPL37
Pfam: Ribosomal protein L37e
InterPro:

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Chain: 2e
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63173 (Residues: 1-70; Coverage: 100%)
Gene name: RPL38
Pfam: Ribosomal L38e protein family
InterPro:

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Chain: 2f
Length: 51 amino acids
Theoretical weight: 6.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62891 (Residues: 1-51; Coverage: 100%)
Gene name: RPL39
Pfam: Ribosomal L39 protein
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Chain: 2g
Length: 128 amino acids
Theoretical weight: 14.76 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62987 (Residues: 1-128; Coverage: 100%)
Gene names: UBA52, UBCEP2
Pfam:
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Chain: 2h
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62945 (Residues: 1-25; Coverage: 100%)
Gene name: RPL41
Pfam: Ribosomal protein L41
InterPro: Large ribosomal subunit protein eS32

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Chain: 2i
Length: 106 amino acids
Theoretical weight: 12.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P83881 (Residues: 1-106; Coverage: 100%)
Gene names: GIG15, MIG6, RPL36A, RPL44
Pfam: Ribosomal protein L44
InterPro:

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Chain: 2j
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61513 (Residues: 1-92; Coverage: 100%)
Gene name: RPL37A
Pfam: Ribosomal L37ae protein family
InterPro:

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Chain: 2k
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46779 (Residues: 1-137; Coverage: 100%)
Gene name: RPL28
Pfam: Ribosomal L28e protein family
InterPro:

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Chain: 2o
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61247 (Residues: 1-264; Coverage: 100%)
Gene names: FTE1, MFTL, RPS3A
Pfam: Ribosomal S3Ae family
InterPro:

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Chain: 2s
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62081 (Residues: 1-194; Coverage: 100%)
Gene name: RPS7
Pfam: Ribosomal protein S7e
InterPro:

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Chain: 2t
Length: 208 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62241 (Residues: 1-208; Coverage: 100%)
Gene names: OK/SW-cl.83, RPS8
Pfam: Ribosomal protein S8e
InterPro:

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Chain: 2u
Length: 165 amino acids
Theoretical weight: 18.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46783 (Residues: 1-165; Coverage: 100%)
Gene name: RPS10
Pfam: Plectin/S10 domain
InterPro:

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Chain: 2w
Length: 145 amino acids
Theoretical weight: 17.08 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62841 (Residues: 1-145; Coverage: 100%)
Gene names: RIG, RPS15
Pfam: Ribosomal protein S19
InterPro:

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Chain: 2x
Length: 146 amino acids
Theoretical weight: 16.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62249 (Residues: 1-146; Coverage: 100%)
Gene name: RPS16
Pfam: Ribosomal protein S9/S16
InterPro:

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Chain: 2y
Length: 135 amino acids
Theoretical weight: 15.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P08708 (Residues: 1-135; Coverage: 100%)
Gene names: RPS17, RPS17L
Pfam: Ribosomal S17
InterPro:

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Chain: 2z
Length: 152 amino acids
Theoretical weight: 17.76 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62269 (Residues: 1-152; Coverage: 100%)
Gene names: D6S218E, RPS18
Pfam: Ribosomal protein S13/S18
InterPro:

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Chain: 20
Length: 145 amino acids
Theoretical weight: 16.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P39019 (Residues: 1-145; Coverage: 100%)
Gene name: RPS19
Pfam: Ribosomal protein S19e
InterPro:

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Chain: 3A
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63220 (Residues: 1-83; Coverage: 100%)
Gene name: RPS21
Pfam: Ribosomal protein S21e
InterPro:

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Chain: 3B
Length: 143 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62266 (Residues: 1-143; Coverage: 100%)
Gene name: RPS23
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: 3C
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62854 (Residues: 1-115; Coverage: 100%)
Gene name: RPS26
Pfam: Ribosomal protein S26e
InterPro:

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Chain: 3D
Length: 69 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62857 (Residues: 1-69; Coverage: 100%)
Gene name: RPS28
Pfam: Ribosomal protein S28e
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Chain: 3H
Length: 249 amino acids
Theoretical weight: 28.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62753 (Residues: 1-249; Coverage: 100%)
Gene names: OK/SW-cl.2, RPS6
Pfam: Ribosomal protein S6e
InterPro:

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Chain: 3J
Length: 132 amino acids
Theoretical weight: 14.55 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25398 (Residues: 1-132; Coverage: 100%)
Gene name: RPS12
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
InterPro:

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Chain: 3L
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62263 (Residues: 1-151; Coverage: 100%)
Gene names: PRO2640, RPS14
Pfam: Ribosomal protein S11
InterPro:

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Chain: 3M
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62244 (Residues: 1-130; Coverage: 100%)
Gene names: OK/SW-cl.82, RPS15A
Pfam: Ribosomal protein S8
InterPro:

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Chain: 3N
Length: 133 amino acids
Theoretical weight: 15.46 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62847 (Residues: 1-133; Coverage: 100%)
Gene name: RPS24
Pfam: Ribosomal protein S24e
InterPro:

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Chain: 3O
Length: 125 amino acids
Theoretical weight: 13.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62851 (Residues: 1-125; Coverage: 100%)
Gene name: RPS25
Pfam: S25 ribosomal protein
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Chain: 3P
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P42677 (Residues: 1-84; Coverage: 100%)
Gene names: MPS1, RPS27
Pfam: Ribosomal protein S27
InterPro:

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Chain: 3Q
Length: 59 amino acids
Theoretical weight: 6.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62861 (Residues: 75-133; Coverage: 44%)
Gene name: FAU
Pfam: Ribosomal protein S30
InterPro: Small ribosomal subunit protein eS30

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Name: 28S ribosomal RNA
Representative chains: 1A
Length: 5070 nucleotides
Theoretical weight: 1640.18 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: 1B
Length: 121 nucleotides
Theoretical weight: 39 KDa
Rfam: 5S ribosomal RNA

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Name: 5.8S ribosomal RNA
Representative chains: 1C
Length: 157 nucleotides
Theoretical weight: 50.45 KDa
Rfam: 5.8S ribosomal RNA

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Name: 18S Ribosomal RNA
Representative chains: 2m
Length: 1869 nucleotides
Theoretical weight: 602.75 KDa
Rfam: Eukaryotic small subunit ribosomal RNA

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Name: Messenger RNA
Representative chains: zv
Length: 6 nucleotides
Theoretical weight: 1.83 KDa

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Name: P-site tRNA
Representative chains: zy
Length: 75 nucleotides
Theoretical weight: 24.19 KDa
Rfam: tRNA

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Name: HCV-IRES RNA
Representative chains: zz
Length: 290 nucleotides
Theoretical weight: 93.49 KDa
Rfam: Hepatitis C virus internal ribosome entry site

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