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Structure of membrane protein

Electron Microscopy
3.52Å resolution
PDB entry 6k1h coloured by chain, front view.
PDB entry 6k1h coloured by chain, side view.
PDB entry 6k1h coloured by chain, top view.
Source organisms:
Assembly composition:
hetero hexamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: PTS system mannose-specific
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Multimeric state: hetero hexamer
Accessible surface area: 62342.69 Å2
Buried surface area: 36326.48 Å2
Dissociation area: 2,798.54 Å2
Dissociation energy (ΔGdiss): 20.32 kcal/mol
Dissociation entropy (TΔSdiss): 30.2 kcal/mol
Symmetry number: 3
PDBe Complex ID: PDB-CPX-159668

Macromolecules

PTS system mannose-specific EIID component
Chains: C, F, Z
Length: 283 amino acids
Theoretical weight: 30.98 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P69805 (Residues: 1-283; Coverage: 100%)
Gene names: JW1808, b1819, gptB, manZ, ptsM
Pfam: PTS system mannose/fructose/sorbose family IID component
InterPro: Phosphotransferase system, mannose/fructose/sorbose family IID component
PTS system mannose-specific EIID component in PDB entry 6k1h, assembly 1, front view.
PTS system mannose-specific EIID component in PDB entry 6k1h, assembly 1, side view.
PTS system mannose-specific EIID component in PDB entry 6k1h, assembly 1, top view.
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PTS system mannose-specific EIIC component
Chains: B, E, Y
Length: 274 amino acids
Theoretical weight: 28.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P69801 (Residues: 1-266; Coverage: 100%)
Gene names: JW1807, b1818, manY, pel, ptsP
Pfam: PTS system sorbose-specific iic component
InterPro: Phosphotransferase system, mannose/fructose/sorbose family, IIC subunit
PTS system mannose-specific EIIC component in PDB entry 6k1h, assembly 1, front view.
PTS system mannose-specific EIIC component in PDB entry 6k1h, assembly 1, side view.
PTS system mannose-specific EIIC component in PDB entry 6k1h, assembly 1, top view.
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