› 6l7v

X-ray diffraction
2.2Å resolution

Crystal structure of Cet1 from Trypanosoma cruzi in complex with tripolyphosphate, manganese and iodide ions.

Released:
DOI: 10.2210/pdb6l7v/pdb
PDB entry 6l7v coloured by chain, front view.
PDB entry 6l7v coloured by chain, side view.
PDB entry 6l7v coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Primary publication:
Crystal structures of the RNA triphosphatase from Trypanosoma cruzi provide insights into how it recognizes the 5'-end of the RNA substrate.
Takagi Y, Kuwabara N, Dang TT, Furukawa K, Ho CK
J Biol Chem 295 9076-9086 (2020)
PMID: 32381506

Function and Biology Details

Reaction catalysed: 
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-559802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA 5'-phosphatase Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.8 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4E2I1 (Residues: 18-243; Coverage: 82%)
Gene name: Tc00.1047053508479.390
Sequence domains: mRNA capping enzyme, beta chain

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand 3PO 1 x 3PO
Ligand IOD 7 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P3221
Unit cell:
a: 67.266Å b: 67.266Å c: 76.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.261
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Enzymatic Assays to Explore Viral mRNA Capping Machinery.
Kasprzyk et al. (2021)
1 other article

2 mentions without citation

Biochemical and biophysical analyses of hypoxia sensing prolyl hydroxylases from Dictyostelium discoideum and Toxoplasma gondii.
Liu et al. (2020)
1 more