› 6lhp

Electron Microscopy
3.3Å resolution

The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fab 14B10

Released:
DOI: 10.2210/pdb6lhp/pdb
PDB entry 6lhp coloured by chain, front view.
PDB entry 6lhp coloured by chain, side view.
PDB entry 6lhp coloured by chain, top view.
Source organisms:
Primary publication:
Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16.
He M, Xu L, Zheng Q, Zhu R, Yin Z, Zha Z, Lin Y, Yang L, Huang Y, Ye X, Li S, Hou W, Wu Y, Han J, Liu D, Li Z, Chen Z, Yu H, Que Y, Wang Y, Yan X, Zhang J, Gu Y, Zhou ZH, Cheng T, Li S, Xia N
Cell Host Microbe 27 249-261.e5 (2020)
PMID: 32027857
Related structures: EMD-0894

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212815 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
light chain variable region of Fab 14B10 Chain: L
Molecule details ›
Chain: L
Length: 107 amino acids
Theoretical weight: 11.67 KDa
Source organism: Mus musculus
heavy chain variable region of Fab 14B10 Chain: H
Molecule details ›
Chain: H
Length: 115 amino acids
Theoretical weight: 12.77 KDa
Source organism: Mus musculus
Capsid protein VP1 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.11 KDa
Source organism: Coxsackievirus A16
UniProt:
  • Canonical: Q9QF31 (Residues: 566-862; Coverage: 14%)
Sequence domains: Picornavirus coat protein
Capsid protein VP2 Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 27.56 KDa
Source organism: Coxsackievirus A16
UniProt:
  • Canonical: Q9QF31 (Residues: 70-323; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Capsid protein VP3 Chain: C
Molecule details ›
Chain: C
Length: 242 amino acids
Theoretical weight: 26.65 KDa
Source organism: Coxsackievirus A16
UniProt:
  • Canonical: Q9QF31 (Residues: 324-565; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: D
Molecule details ›
Chain: D
Length: 69 amino acids
Theoretical weight: 7.55 KDa
Source organism: Coxsackievirus A16
UniProt:
  • Canonical: Q9QF31 (Residues: 1-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)

Ligands and Environments

1 bound ligand:
Ligand SPH 1 x SPH
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-0894

Quick links

Citations

14 citation in other articles

Cryo-EM structures reveal the molecular basis of receptor-initiated coxsackievirus uncoating.
Xu et al. (2021)
13 more

1 mention without citation

Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16.
He et al. (2020)