6lq8

X-ray diffraction
1.85Å resolution

Crystal Structure of E447A Acyl-CoA Dehydrogenase FadE5 mutant from Mycobacteria smegmatis in complex with C22CoA

Released:
Model geometry
Fit model/data

Function and Biology Details

Reactions catalysed:
A short-chain acyl-CoA + electron-transfer flavoprotein = a short-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein
A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein
Long-chain-acyl-CoA + electron-transfer flavoprotein = long-chain-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-174725 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Broad-specificity linear acyl-CoA dehydrogenase FadE5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 612 amino acids
Theoretical weight: 66.63 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3L887 (Residues: 1-611; Coverage: 100%)
Gene names: MSMEG_0406, MSMEI_0396, fadE5
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 98.082Å b: 205.723Å c: 74.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.195
Expression system: Escherichia coli BL21(DE3)