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Crystal structure of AnkG/beta2-spectrin complex

X-ray diffraction
3.312Å resolution
PDB entry 6m3p coloured by chain, front view.
PDB entry 6m3p coloured by chain, side view.
PDB entry 6m3p coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Spectrin beta chain, non-erythrocytic 1 and Ankyrin-3
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Multimeric state: hetero dimer
Accessible surface area: 37587.49 Å2
Buried surface area: 1866.19 Å2
Dissociation area: 933.09 Å2
Dissociation energy (ΔGdiss): -1.84 kcal/mol
Dissociation entropy (TΔSdiss): 13.8 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-130706
Assembly 2
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Assembly Name: Spectrin beta chain, non-erythrocytic 1 and Ankyrin-3
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Multimeric state: hetero dimer
Accessible surface area: 37271.71 Å2
Buried surface area: 1888.95 Å2
Dissociation area: 944.47 Å2
Dissociation energy (ΔGdiss): -1.46 kcal/mol
Dissociation entropy (TΔSdiss): 13.79 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-130706

Macromolecules

Ankyrin-3
Chains: C, E
Length: 491 amino acids
Theoretical weight: 54.56 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O70511 (Residues: 975-1465; Coverage: 19%)
Gene name: Ank3
Pfam:
InterPro:
Ankyrin-3 in PDB entry 6m3p, assembly 1, front view.
Ankyrin-3 in PDB entry 6m3p, assembly 1, side view.
Ankyrin-3 in PDB entry 6m3p, assembly 1, top view.
PDBe-KB: UniProt Coverage View: O70511  
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Spectrin beta chain, non-erythrocytic 1
Chains: A, B
Length: 320 amino acids
Theoretical weight: 37.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62261 (Residues: 1591-1910; Coverage: 14%)
Gene names: Elf, Spnb-2, Spnb2, Sptb2, Sptbn1
Pfam: Spectrin repeat
InterPro:
Spectrin beta chain, non-erythrocytic 1 in PDB entry 6m3p, assembly 1, front view.
Spectrin beta chain, non-erythrocytic 1 in PDB entry 6m3p, assembly 1, side view.
Spectrin beta chain, non-erythrocytic 1 in PDB entry 6m3p, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q62261  
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