› 6nht

Electron Microscopy
2.9Å resolution

Single particle reconstruction of the symmetric core an engineered protein scaffold

Released:
DOI: 10.2210/pdb6nht/pdb
PDB entry 6nht coloured by chain, front view.
PDB entry 6nht coloured by chain, side view.
PDB entry 6nht coloured by chain, top view.
Source organisms:
Primary publication:
A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold.
Liu Y, Huynh DT, Yeates TO
OpenAccess logo Nat Commun 10 1864 (2019)
PMID: 31015551
Related structures: EMD-9373

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167128 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DARP14 - Subunit A with DARPin Chains: A, B, C, D, I, J, K, L, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, I, J, K, L, Q, R, S, T
Length: 319 amino acids
Theoretical weight: 34.72 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
5-carboxymethyl-2-hydroxymuconate isomerase Chains: E, F, G, H, M, N, O, P, U, V, W, X
Molecule details ›
Chains: E, F, G, H, M, N, O, P, U, V, W, X
Length: 131 amino acids
Theoretical weight: 14.35 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I2D8 (Residues: 1-123; Coverage: 100%)
Gene name: PA1966
Sequence domains: 5-carboxymethyl-2-hydroxymuconate isomerase
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-9373
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Advances in protein structure prediction and design.
Kuhlman et al. (2019)
10 more

1 mention without citation

Putting on molecular weight: Enabling cryo-EM structure determination of sub-100-kDa proteins.
Wentinck et al. (2022)