6nzu

Electron Microscopy
3.2Å resolution

Structure of the human frataxin-bound iron-sulfur cluster assembly complex

Released:

Function and Biology Details

Reactions catalysed:
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-167173 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Cysteine desulfurase Chains: A, E
Molecule details ›
Chains: A, E
Length: 403 amino acids
Theoretical weight: 44.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
LYR motif-containing protein 4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 92 amino acids
Theoretical weight: 10.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Acyl carrier protein Chains: C, G
Iron-sulfur cluster assembly enzyme ISCU Chains: D, H
Frataxin mature form Chains: I, J

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-0560
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'