› 6oh6

X-ray diffraction
2.07Å resolution

Crystal structure of (E)-biformene synthase LrdC from Streptomyces sp. strain K155 in complex with Mg and PPi

Released:
DOI: 10.2210/pdb6oh6/pdb
PDB entry 6oh6 coloured by chain, front view.
PDB entry 6oh6 coloured by chain, side view.
PDB entry 6oh6 coloured by chain, top view.
Source organism: Streptomyces sp.
Primary publication:
The structure of (E)-biformene synthase provides insights into the biosynthesis of bacterial bicyclic labdane-related diterpenoids.
Centeno-Leija S, Tapia-Cabrera S, Guzmán-Trampe S, Esquivel B, Esturau-Escofet N, Tierrafría VH, Rodríguez-Sanoja R, Zárate-Romero A, Stojanoff V, Rudiño-Piñera E, Sánchez S, Serrano-Posada H
J Struct Biol 207 29-39 (2019)
PMID: 30981884

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-495210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terpene synthase Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 37.24 KDa
Source organism: Streptomyces sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A158RFK9 (Residues: 1-342; Coverage: 100%)
Sequence domains: Terpene synthase family 2, C-terminal metal binding
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

4 bound ligands:
Ligand PPV 1 x PPV
Ligand MG 3 x MG
Ligand GOL 1 x GOL
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 107.05Å b: 107.05Å c: 88.821Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.233 0.252
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Current understanding and biotechnological application of the bacterial diterpene synthase CotB2.
Driller et al. (2019)
1 more