6p07

Electron Microscopy
3.2Å resolution

Spastin hexamer in complex with substrate

Released:
Source organism: Drosophila melanogaster
Related structures: EMD-20226

Function and Biology Details

Reaction catalysed:
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100960 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spastin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 494 amino acids
Theoretical weight: 54.18 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B4LHJ5 (Residues: 209-696; Coverage: 70%)
Gene names: CG5977, D-spastin, Dmel\CG5977, Dmel_CG5977, Dspastin, Spas, Spastin, spas
Sequence domains:
polyglutamate peptide Chain: G
Molecule details ›
Chain: G
Length: 15 amino acids
Theoretical weight: 1.95 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-20226
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided