› 6ppj

Electron Microscopy
3.5Å resolution

Cryo-EM structure of AdnA(D934A)-AdnB(D1014A) in complex with AMPPNP

Released:
DOI: 10.2210/pdb6ppj/pdb
PDB entry 6ppj coloured by chain, front view.
PDB entry 6ppj coloured by chain, side view.
PDB entry 6ppj coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis
Primary publication:
Structures and single-molecule analysis of bacterial motor nuclease AdnAB illuminate the mechanism of DNA double-strand break resection.
Jia N, Unciuleac MC, Xue C, Greene EC, Patel DJ, Shuman S
Proc Natl Acad Sci U S A 116 24507-24516 (2019)
PMID: 31740608
Related structures: EMD-20440

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106316 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA 3'-5' helicase Chain: B
Molecule details ›
Chain: B
Length: 1095 amino acids
Theoretical weight: 114.74 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QTS0 (Residues: 1-1087; Coverage: 100%)
Gene name: MSMEG_1943
Sequence domains:
DNA 3'-5' helicase Chain: A
Molecule details ›
Chain: A
Length: 1045 amino acids
Theoretical weight: 110.9 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QTR9 (Residues: 1-1045; Coverage: 100%)
Gene name: MSMEG_1941
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SF4 1 x SF4
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-20440
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bacterial phenotypic heterogeneity in DNA repair and mutagenesis.
Vincent et al. (2020)
2 more

2 mentions without citation

Recent Insights into the Structure and Function of Mycobacterial Membrane Proteins Facilitated by Cryo-EM.
Bendre et al. (2021)
1 more