› 6ptn

Electron Microscopy
5.8Å resolution

Structure of Ctf4 trimer in complex with two CMG helicases

Released:
DOI: 10.2210/pdb6ptn/pdb
PDB entry 6ptn coloured by chain, front view.
PDB entry 6ptn coloured by chain, side view.
PDB entry 6ptn coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Ctf4 organizes sister replisomes and Pol α into a replication factory.
Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O'Donnell ME, Li H
OpenAccess logo Elife 8 (2019)
PMID: 31589141
Related structures: EMD-20472

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 25-mer (preferred)
PDBe Complex ID:
PDB-CPX-150192 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
DNA polymerase alpha-binding protein Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 927 amino acids
Theoretical weight: 104.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q01454 (Residues: 1-927; Coverage: 100%)
Gene names: CHL15, CTF4, P9659.7, POB1, YPR135W
Sequence domains:
DNA replication complex GINS protein PSF1 Chains: A, a
Molecule details ›
Chains: A, a
Length: 208 amino acids
Theoretical weight: 24.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12488 (Residues: 1-208; Coverage: 100%)
Gene names: PSF1, PZA208, YD8119.18, YDR013W
Sequence domains: GINS complex protein helical bundle domain
DNA replication complex GINS protein PSF2 Chains: B, b
Molecule details ›
Chains: B, b
Length: 213 amino acids
Theoretical weight: 25.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40359 (Residues: 1-213; Coverage: 100%)
Gene names: HRF213, J1086, PSF2, YJL072C
Sequence domains: GINS complex protein helical bundle domain
DNA replication complex GINS protein PSF3 Chains: C, c
Molecule details ›
Chains: C, c
Length: 194 amino acids
Theoretical weight: 21.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12146 (Residues: 1-194; Coverage: 100%)
Gene names: PSF3, YOL146W
Sequence domains:
DNA replication complex GINS protein SLD5 Chains: D, d
Molecule details ›
Chains: D, d
Length: 294 amino acids
Theoretical weight: 33.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03406 (Residues: 1-294; Coverage: 100%)
Gene names: SLD5, YDR489W
Sequence domains:
Cell division control protein 45 Chains: H, h
Molecule details ›
Chains: H, h
Length: 650 amino acids
Theoretical weight: 74.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q08032 (Residues: 1-650; Coverage: 100%)
Gene names: CDC45, L8004.11, SLD4, YLR103C
Sequence domains: CDC45-like protein
DNA replication licensing factor MCM2 Chains: 2, i
Molecule details ›
Chains: 2, i
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM3 Chains: 3, j
Molecule details ›
Chains: 3, j
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chains: 4, k
Molecule details ›
Chains: 4, k
Length: 933 amino acids
Theoretical weight: 105.14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30665 (Residues: 1-933; Coverage: 100%)
Gene names: CDC54, HCD21, MCM4, YP9531.13, YPR019W
Sequence domains:
Minichromosome maintenance protein 5 Chains: 5, l
Molecule details ›
Chains: 5, l
Length: 775 amino acids
Theoretical weight: 86.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29496 (Residues: 1-775; Coverage: 100%)
Gene names: CDC46, L9328.1, MCM5, YLR274W
Sequence domains:
DNA replication licensing factor MCM6 Chains: 6, m
Molecule details ›
Chains: 6, m
Length: 1017 amino acids
Theoretical weight: 113.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53091 (Residues: 1-1017; Coverage: 100%)
Gene names: MCM6, YGL201C
Sequence domains:
DNA replication licensing factor MCM7 Chains: 7, n
Molecule details ›
Chains: 7, n
Length: 845 amino acids
Theoretical weight: 95.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-845; Coverage: 100%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 6 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.8Å
Relevant EMDB volumes: EMD-20472
Expression system: Saccharomyces cerevisiae

Quick links

Citations

8 review citations

Mechanisms of hexameric helicases.
Fernandez et al. (2021)
7 more

1 mention without citation

Ctf4 organizes sister replisomes and Pol α into a replication factory.
Yuan et al. (2019)