Structure analysis

Cryo-EM structure of MLL1 in complex with RbBP5 and WDR5 bound to the nucleosome

Electron Microscopy
4.4Å resolution
Source organisms:
Assembly composition:
hetero tridecamer (preferred)
Entry contents: 7 distinct polypeptide molecules
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tridecamer
Accessible surface area: 103561.0 Å2
Buried surface area: 59460.61 Å2
Dissociation area: 1,984.74 Å2
Dissociation energy (ΔGdiss): -20.88 kcal/mol
Dissociation entropy (TΔSdiss): 41.85 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-120471

Macromolecules

Chain: A
Length: 538 amino acids
Theoretical weight: 59.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15291 (Residues: 2-538; Coverage: 100%)
Gene names: RBBP5, RBQ3
Pfam: WD domain, G-beta repeat
InterPro:
PDBe-KB: UniProt Coverage View: Q15291  
 
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Chain: B
Length: 313 amino acids
Theoretical weight: 34.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 22-334; Coverage: 94%)
Gene names: BIG3, WDR5
Pfam: WD domain, G-beta repeat
InterPro:
PDBe-KB: UniProt Coverage View: P61964  
 
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Chain: C
Length: 209 amino acids
Theoretical weight: 24.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03164 (Residues: 3762-3969; Coverage: 5%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
Pfam: SET domain
InterPro:
PDBe-KB: UniProt Coverage View: Q03164  
 
UniProt

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Chains: G, K
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 1-136; Coverage: 100%)
Pfam: Core histone H2A/H2B/H3/H4
InterPro:
PDBe-KB: UniProt Coverage View: P84233  
 
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Chains: H, L
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Pfam: Centromere kinetochore component CENP-T histone fold
InterPro:
PDBe-KB: UniProt Coverage View: P62799  
 
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Chains: I, M
Length: 129 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 2-130; Coverage: 99%)
Pfam:
InterPro:
PDBe-KB: UniProt Coverage View: P06897  
 
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Chains: J, N
Length: 123 amino acids
Theoretical weight: 13.66 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-126; Coverage: 97%)
Pfam: Core histone H2A/H2B/H3/H4
InterPro:
PDBe-KB: UniProt Coverage View: P02281  
 
UniProt

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Name: DNA (146-MER)
Representative chains: O
Length: 147 nucleotides
Theoretical weight: 45.14 KDa
 
Chains

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Name: DNA (146-MER)
Representative chains: P
Length: 147 nucleotides
Theoretical weight: 45.61 KDa
 
Chains

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