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Crystal structure of VASH 2 in complex with SVBP

X-ray diffraction
2.09Å resolution
PDB entry 6qby coloured by chain, front view.
PDB entry 6qby coloured by chain, side view.
PDB entry 6qby coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Small vasohibin-binding protein and Tubulinyl-Tyr carboxypeptidase 2
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Multimeric state: hetero dimer
Accessible surface area: 13745.36 Å2
Buried surface area: 2381.68 Å2
Dissociation area: 1,190.84 Å2
Dissociation energy (ΔGdiss): 11.42 kcal/mol
Dissociation entropy (TΔSdiss): 9.67 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-183206
Assembly 2
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Assembly Name: Small vasohibin-binding protein and Tubulinyl-Tyr carboxypeptidase 2
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Multimeric state: hetero dimer
Accessible surface area: 13927.97 Å2
Buried surface area: 2543.79 Å2
Dissociation area: 1,271.89 Å2
Dissociation energy (ΔGdiss): 11.32 kcal/mol
Dissociation entropy (TΔSdiss): 9.99 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-183206

Macromolecules

Tubulinyl-Tyr carboxypeptidase 2
Chains: A, C
Length: 257 amino acids
Theoretical weight: 30 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q86V25 (Residues: 40-295; Coverage: 72%)
Gene names: VASH2, VASHL
Pfam: Vasohibin
InterPro: Tubulinyl-Tyr carboxypeptidase
Tubulinyl-Tyr carboxypeptidase 2 in PDB entry 6qby, assembly 1, front view.
Tubulinyl-Tyr carboxypeptidase 2 in PDB entry 6qby, assembly 1, side view.
Tubulinyl-Tyr carboxypeptidase 2 in PDB entry 6qby, assembly 1, top view.
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Small vasohibin-binding protein
Chains: B, D
Length: 74 amino acids
Theoretical weight: 8.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8N300 (Residues: 1-66; Coverage: 100%)
Gene names: CCDC23, SVBP
Pfam: Coiled-coil domain-containing protein 23
InterPro: Small vasohibin-binding protein
Small vasohibin-binding protein in PDB entry 6qby, assembly 1, front view.
Small vasohibin-binding protein in PDB entry 6qby, assembly 1, side view.
Small vasohibin-binding protein in PDB entry 6qby, assembly 1, top view.
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