› 6rxf

X-ray diffraction
2.4Å resolution

Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Phosphohistidine Intermediate

Released:
DOI: 10.2210/pdb6rxf/pdb
PDB entry 6rxf coloured by chain, front view.
PDB entry 6rxf coloured by chain, side view.
PDB entry 6rxf coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Primary publication:
Snapshots during the catalytic cycle of a histidine acid phytase reveal an induced-fit structural mechanism.
Acquistapace IM, Zi Etek MA, Li AWH, Salmon M, Kühn I, Bedford MR, Brearley CA, Hemmings AM
OpenAccess logo J Biol Chem 295 17724-17737 (2020)
PMID: 33454010

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110476 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histidine acid phosphatase Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 56.49 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli
UniProt:
  • Canonical: B7GTV0 (Residues: 33-545; Coverage: 87%)
Gene name: Blon_0263
Sequence domains: Histidine phosphatase superfamily (branch 2)
Histidine acid phosphatase Chain: B
Molecule details ›
Chain: B
Length: 519 amino acids
Theoretical weight: 56.57 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli
UniProt:
  • Canonical: B7GTV0 (Residues: 33-545; Coverage: 87%)
Gene name: Blon_0263
Sequence domains: Histidine phosphatase superfamily (branch 2)

Ligands and Environments

1 bound ligand:
Ligand ZN 17 x ZN
1 modified residue:
Ligand NEP 1 x NEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 55.508Å b: 73.118Å c: 88.987Å
α: 71.3° β: 72.99° γ: 78.99°
R-values:
R R work R free
0.182 0.182 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Inositol Phosphates and Retroviral Assembly: A Cellular Perspective.
Ricaña et al. (2021)
1 more

1 mention without citation

Snapshots during the catalytic cycle of a histidine acid phytase reveal an induced-fit structural mechanism.
Acquistapace et al. (2020)