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Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Phosphohistidine Intermediate

X-ray diffraction
2.397Å resolution
PDB entry 6rxf coloured by chain, front view.
PDB entry 6rxf coloured by chain, side view.
PDB entry 6rxf coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Assembly composition:
monomeric (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: Histidine acid phosphatase
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Multimeric state: monomeric
Accessible surface area: 21108.84 Å2
Buried surface area: 767.36 Å2
Dissociation area: 18.65 Å2
Dissociation energy (ΔGdiss): 15.13 kcal/mol
Dissociation entropy (TΔSdiss): -0.21 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-110476
Assembly 2 (preferred)
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Assembly Name: Histidine acid phosphatase
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Multimeric state: monomeric
Accessible surface area: 21063.89 Å2
Buried surface area: 972.85 Å2
Dissociation area: 67.83 Å2
Dissociation energy (ΔGdiss): 39.21 kcal/mol
Dissociation entropy (TΔSdiss): -0.2 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-110476

Macromolecules

Histidine acid phosphatase
Chain: A
Length: 519 amino acids
Theoretical weight: 56.49 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli
UniProt:
  • Canonical: B7GTV0 (Residues: 33-545; Coverage: 87%)
Gene name: Blon_0263
Pfam: Histidine phosphatase superfamily (branch 2)
InterPro:
Multiple inositol polyphosphate phosphatase 1 in PDB entry 6rxf, assembly 1, front view.
Multiple inositol polyphosphate phosphatase 1 in PDB entry 6rxf, assembly 1, side view.
Multiple inositol polyphosphate phosphatase 1 in PDB entry 6rxf, assembly 1, top view.
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Histidine acid phosphatase
Chain: B
Length: 519 amino acids
Theoretical weight: 56.57 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli
UniProt:
  • Canonical: B7GTV0 (Residues: 33-545; Coverage: 87%)
Gene name: Blon_0263
Pfam: Histidine phosphatase superfamily (branch 2)
InterPro:
Multiple inositol polyphosphate phosphatase 1 in PDB entry 6rxf, assembly 2, front view.
Multiple inositol polyphosphate phosphatase 1 in PDB entry 6rxf, assembly 2, side view.
Multiple inositol polyphosphate phosphatase 1 in PDB entry 6rxf, assembly 2, top view.
Molecule details ›

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