› 6s6v

Electron Microscopy
3.5Å resolution

Resting state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ATPgS

Released:
DOI: 10.2210/pdb6s6v/pdb
PDB entry 6s6v coloured by chain, front view.
PDB entry 6s6v coloured by chain, side view.
PDB entry 6s6v coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanism of DNA End Sensing and Processing by the Mre11-Rad50 Complex.
Käshammer L, Saathoff JH, Lammens K, Gut F, Bartho J, Alt A, Kessler B, Hopfner KP
Mol Cell 76 382-394.e6 (2019)
PMID: 31492634
Related structures: EMD-10107

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclease SbcCD subunit D Chains: A, B
Molecule details ›
Chains: A, B
Length: 407 amino acids
Theoretical weight: 45.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG76 (Residues: 1-400; Coverage: 100%)
Gene names: JW0388, b0398, sbcD, yajA
Sequence domains:
Nuclease SbcCD subunit C Chains: C, D
Molecule details ›
Chains: C, D
Length: 1048 amino acids
Theoretical weight: 118.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13458 (Residues: 1-1048; Coverage: 100%)
Gene names: JW0387, b0397, rmuA, sbcC
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand AGS 2 x AGS
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-10107
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Molecular and Structural Basis of DNA Sensors in Antiviral Innate Immunity.
Zahid et al. (2020)
15 more

7 mentions without citation

Functional and structural insights into the MRX/MRN complex, a key player in recognition and repair of DNA double-strand breaks.
Tisi et al. (2020)
6 more