› 6s8b

Electron Microscopy
2.41Å resolution

Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1

Released:
DOI: 10.2210/pdb6s8b/pdb
PDB entry 6s8b coloured by chain, front view.
PDB entry 6s8b coloured by chain, side view.
PDB entry 6s8b coloured by chain, top view.
Source organism: Sulfolobus islandicus REY15A
Primary publication:
Structures of the Cmr-β Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas.
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell 79 741-757.e7 (2020)
PMID: 32730741
Related structures: EMD-10117

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-123246 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (9 distinct):
CRISPR type III-B/RAMP module-associated protein Cmr5 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 155 amino acids
Theoretical weight: 17.96 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX5 (Residues: 1-155; Coverage: 100%)
Gene name: SiRe_0601
CRISPR-associated RAMP protein, Cmr4 family Chains: D, E, F, G
Molecule details ›
Chains: D, E, F, G
Length: 286 amino acids
Theoretical weight: 32.32 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX6 (Residues: 1-286; Coverage: 100%)
Gene name: SiRe_0602
Sequence domains: RAMP superfamily
CRISPR-associated protein, Cmr3 family Chain: H
Molecule details ›
Chain: H
Length: 313 amino acids
Theoretical weight: 36.01 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX1 (Residues: 1-313; Coverage: 100%)
Gene name: SiRe_0597
Sequence domains: CRISPR-associated protein (Cas_Cmr3)
CRISPR-associated RAMP protein, Cmr6 family Chain: I
Molecule details ›
Chain: I
Length: 296 amino acids
Theoretical weight: 33.95 KDa
Source organism: Sulfolobus islandicus REY15A
Expression system: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX3 (Residues: 1-283; Coverage: 100%)
Gene name: SiRe_0599
Sequence domains: RAMP superfamily
CRISPR-associated RAMP protein, Cmr1 family Chain: J
Molecule details ›
Chain: J
Length: 476 amino acids
Theoretical weight: 55.39 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX4 (Residues: 1-471; Coverage: 100%)
Gene name: SiRe_0600
Sequence domains: RAMP superfamily
CRISPR-associated protein Cmrx Chains: L, M, N, O, P, Q, R, S, T, W, X, l, m, n, o, p, q, r, s, t, w, x
Molecule details ›
Chains: L, M, N, O, P, Q, R, S, T, W, X, l, m, n, o, p, q, r, s, t, w, x
Length: 174 amino acids
Theoretical weight: 19.71 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX7 (Residues: 1-174; Coverage: 100%)
Gene name: SiRe_0603
Sequence domains: CRISPR system CMR subunit Cmr7 1
CRISPR-associated protein Cmr2 N-terminal domain-containing protein Chain: K
Molecule details ›
Chain: K
Length: 1037 amino acids
Theoretical weight: 120.86 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX2 (Residues: 1-1037; Coverage: 100%)
Gene name: SiRe_0598
Sequence domains:
Cognate target RNA (46-mer) Chain: U
Molecule details ›
Chain: U
Length: 46 nucleotides
Theoretical weight: 14.7 KDa
crRNA Chain: V
Molecule details ›
Chain: V
Length: 51 nucleotides
Theoretical weight: 16.41 KDa

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand ANP 2 x ANP
Ligand MN 3 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.41Å
Relevant EMDB volumes: EMD-10117
Expression system: Sulfolobus islandicus REY15A

Quick links

Citations

7 review citations

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.
Jia et al. (2021)
6 more

1 mention without citation

Residue-wise local quality estimation for protein models from cryo-EM maps.
Terashi et al. (2022)