› › Structure analysis

Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1

Electron Microscopy
2.41Å resolution
PDB entry 6s8b coloured by chain, front view.
PDB entry 6s8b coloured by chain, side view.
PDB entry 6s8b coloured by chain, top view.
Source organism: Sulfolobus islandicus REY15A
Assembly composition:
hetero 35-mer (preferred)
Entry contents: 7 distinct polypeptide molecules
2 distinct RNA molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Multimeric state: hetero 35-mer
Accessible surface area: 274184.36 Å2
Buried surface area: 124206.99 Å2
Dissociation area: 3,944.82 Å2
Dissociation energy (ΔGdiss): -13.68 kcal/mol
Dissociation entropy (TΔSdiss): 58.74 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-123246

Macromolecules

CRISPR type III-B/RAMP module-associated protein Cmr5
Chains: A, B, C
Length: 155 amino acids
Theoretical weight: 17.96 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX5 (Residues: 1-155; Coverage: 100%)
Gene name: SiRe_0601
InterPro:
CRISPR type III-B/RAMP module-associated protein Cmr5 in PDB entry 6s8b, assembly 1, front view.
CRISPR type III-B/RAMP module-associated protein Cmr5 in PDB entry 6s8b, assembly 1, side view.
CRISPR type III-B/RAMP module-associated protein Cmr5 in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR-associated RAMP protein, Cmr4 family
Chains: D, E, F, G
Length: 286 amino acids
Theoretical weight: 32.32 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX6 (Residues: 1-286; Coverage: 100%)
Gene name: SiRe_0602
Pfam: RAMP superfamily
InterPro:
CRISPR-associated RAMP protein, Cmr4 family in PDB entry 6s8b, assembly 1, front view.
CRISPR-associated RAMP protein, Cmr4 family in PDB entry 6s8b, assembly 1, side view.
CRISPR-associated RAMP protein, Cmr4 family in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR-associated protein, Cmr3 family
Chain: H
Length: 313 amino acids
Theoretical weight: 36.01 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX1 (Residues: 1-313; Coverage: 100%)
Gene name: SiRe_0597
Pfam: CRISPR-associated protein (Cas_Cmr3)
InterPro:
CRISPR-associated protein, Cmr3 family in PDB entry 6s8b, assembly 1, front view.
CRISPR-associated protein, Cmr3 family in PDB entry 6s8b, assembly 1, side view.
CRISPR-associated protein, Cmr3 family in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR-associated RAMP protein, Cmr6 family
Chain: I
Length: 296 amino acids
Theoretical weight: 33.95 KDa
Source organism: Sulfolobus islandicus REY15A
Expression system: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX3 (Residues: 1-283; Coverage: 100%)
Gene name: SiRe_0599
Pfam: RAMP superfamily
InterPro:
CRISPR-associated RAMP protein, Cmr6 family in PDB entry 6s8b, assembly 1, front view.
CRISPR-associated RAMP protein, Cmr6 family in PDB entry 6s8b, assembly 1, side view.
CRISPR-associated RAMP protein, Cmr6 family in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR-associated RAMP protein, Cmr1 family
Chain: J
Length: 476 amino acids
Theoretical weight: 55.39 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX4 (Residues: 1-471; Coverage: 100%)
Gene name: SiRe_0600
Pfam: RAMP superfamily
InterPro:
CRISPR-associated RAMP protein, Cmr1 family in PDB entry 6s8b, assembly 1, front view.
CRISPR-associated RAMP protein, Cmr1 family in PDB entry 6s8b, assembly 1, side view.
CRISPR-associated RAMP protein, Cmr1 family in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR-associated protein Cmrx
Chains: L, M, N, O, P, Q, R, S, T, W, X, l, m, n, o, p, q, r, s, t, w, x
Length: 174 amino acids
Theoretical weight: 19.71 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX7 (Residues: 1-174; Coverage: 100%)
Gene name: SiRe_0603
Pfam: CRISPR system CMR subunit Cmr7 1
InterPro: CRISPR system CMR subunit Cmr7 1
CRISPR-associated protein Cmrx in PDB entry 6s8b, assembly 1, front view.
CRISPR-associated protein Cmrx in PDB entry 6s8b, assembly 1, side view.
CRISPR-associated protein Cmrx in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR-associated protein, Cmr2 family
Chain: K
Length: 1037 amino acids
Theoretical weight: 120.86 KDa
Source organism: Sulfolobus islandicus REY15A
UniProt:
  • Canonical: F0NDX2 (Residues: 1-1037; Coverage: 100%)
Gene name: SiRe_0598
Pfam:
InterPro:
CRISPR-associated protein Cmr2 N-terminal domain-containing protein in PDB entry 6s8b, assembly 1, front view.
CRISPR-associated protein Cmr2 N-terminal domain-containing protein in PDB entry 6s8b, assembly 1, side view.
CRISPR-associated protein Cmr2 N-terminal domain-containing protein in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar proteins

RNA chain U
Name: Cognate target RNA (46-mer)
Representative chains: U
Length: 46 nucleotides
Theoretical weight: 14.7 KDa
Cognate target RNA (46-mer) in PDB entry 6s8b, assembly 1, front view.
Cognate target RNA (46-mer) in PDB entry 6s8b, assembly 1, side view.
Cognate target RNA (46-mer) in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar RNA

RNA chain V
Name: crRNA
Representative chains: V
Length: 51 nucleotides
Theoretical weight: 16.41 KDa
crRNA in PDB entry 6s8b, assembly 1, front view.
crRNA in PDB entry 6s8b, assembly 1, side view.
crRNA in PDB entry 6s8b, assembly 1, top view.
Molecule details ›

Search similar RNA

Quick links