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Structure of the Rap conjugation gene regulator of the plasmid pLS20 in complex with the Phr* peptide

X-ray diffraction
2.45Å resolution
PDB entry 6t46 coloured by chain, front view.
PDB entry 6t46 coloured by chain, side view.
PDB entry 6t46 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. natto
Assembly composition:
hetero octamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Response regulator aspartate phosphatase and Phr family secreted Rap phosphatase inhibitor
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Multimeric state: hetero octamer
Accessible surface area: 62432.03 Å2
Buried surface area: 17796.85 Å2
Dissociation area: 2,527.51 Å2
Dissociation energy (ΔGdiss): 34.29 kcal/mol
Dissociation entropy (TΔSdiss): 15.64 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-123232
Assembly 2
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Assembly Name: Response regulator aspartate phosphatase and Phr family secreted Rap phosphatase inhibitor
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Multimeric state: hetero octamer
Accessible surface area: 63955.24 Å2
Buried surface area: 16138.2 Å2
Dissociation area: 1,322.74 Å2
Dissociation energy (ΔGdiss): 10.94 kcal/mol
Dissociation entropy (TΔSdiss): 12.17 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-123232

Macromolecules

Response regulator aspartate phosphatase
Chains: A, C, E, G
Length: 376 amino acids
Theoretical weight: 44.5 KDa
Source organism: Bacillus subtilis subsp. natto
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E9RIY6 (Residues: 1-368; Coverage: 100%)
Gene name: rapA
Pfam:
InterPro:
Response regulator aspartate phosphatase in PDB entry 6t46, assembly 1, front view.
Response regulator aspartate phosphatase in PDB entry 6t46, assembly 1, side view.
Response regulator aspartate phosphatase in PDB entry 6t46, assembly 1, top view.
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Phr family secreted Rap phosphatase inhibitor
Chains: B, D, F, H
Length: 44 amino acids
Theoretical weight: 4.64 KDa
Source organism: Bacillus subtilis subsp. natto
Expression system: Not provided
UniProt:
  • Canonical: E9RIY7 (Residues: 1-44; Coverage: 100%)
Phr family secreted Rap phosphatase inhibitor in PDB entry 6t46, assembly 1, front view.
Phr family secreted Rap phosphatase inhibitor in PDB entry 6t46, assembly 1, side view.
Phr family secreted Rap phosphatase inhibitor in PDB entry 6t46, assembly 1, top view.
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