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Crystal structure of the SVS_A2 protein (A224I mutant) from ancestral sequence reconstruction at 2.6 A resolution

X-ray diffraction
2.6Å resolution
PDB entry 6thu coloured by chain, front view.
PDB entry 6thu coloured by chain, side view.
PDB entry 6thu coloured by chain, top view.
Source organism: Streptomyces sp. CWA1
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Svs_as10 variant
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Multimeric state: homo dimer
Accessible surface area: 25247.14 Å2
Buried surface area: 3757.8 Å2
Dissociation area: 1,878.9 Å2
Dissociation energy (ΔGdiss): 12.76 kcal/mol
Dissociation entropy (TΔSdiss): 13.93 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-167510

Macromolecules

SVS_AS10 variant
Chains: A, B
Length: 361 amino acids
Theoretical weight: 40.52 KDa
Source organism: Streptomyces sp. CWA1
Expression system: Escherichia coli
InterPro:
SVS_AS10 variant in PDB entry 6thu, assembly 1, front view.
SVS_AS10 variant in PDB entry 6thu, assembly 1, side view.
SVS_AS10 variant in PDB entry 6thu, assembly 1, top view.
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