6tko

Electron Microscopy
3.3Å resolution

Phosphorylated turkey beta1 adrenoceptor with bound agonist formoterol coupled to arrestin-2 in lipid nanodisc.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-209328 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-1 adrenergic receptor Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.28 KDa
Source organism: Meleagris gallopavo
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P07700 (Residues: 32-357; Coverage: 62%)
Gene name: ADRB1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Beta-arrestin-1 Chain: B
Molecule details ›
Chain: B
Length: 418 amino acids
Theoretical weight: 47.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49407 (Residues: 1-418; Coverage: 100%)
Gene names: ARR1, ARRB1
Sequence domains:
Fab30 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.51 KDa
Source organism: Phage display vector pTDisp
Expression system: Escherichia coli
Fab30 light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.44 KDa
Source organism: Phage display vector pTDisp
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-10515
Expression systems:
  • Trichoplusia ni
  • Escherichia coli