› 6u4o

X-ray diffraction
1.85Å resolution

Crystal structure of recombinant class II fumarase from Schistosoma mansoni

Released:
DOI: 10.2210/pdb6u4o/pdb
PDB entry 6u4o coloured by chain, front view.
PDB entry 6u4o coloured by chain, side view.
PDB entry 6u4o coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Characterization of class II fumarase from Schistosoma mansoni provides the molecular basis for selective inhibition.
Cardoso IA, de Souza AKL, Burgess AMG, Chalmers IW, Hoffmann KF, Nonato MC
Int J Biol Macromol 175 406-421 (2021)
PMID: 33549669

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
fumarate hydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 488 amino acids
Theoretical weight: 53.83 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3Q0KQY7 (Residues: 1-488; Coverage: 100%)
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand LMR 4 x LMR
Ligand GOL 4 x GOL
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 180.65Å b: 67.86Å c: 187.27Å
α: 90° β: 118.61° γ: 90°
R-values:
R R work R free
0.194 0.192 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Schistosoma mansoni α-N-acetylgalactosaminidase (SmNAGAL) regulates coordinated parasite movement and egg production.
Hulme et al. (2022)
1 more