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Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development.

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Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW
OpenAccess logo mBio 11 (2020)
Related entries: 6v39, 6v3a, 6v3b, 6v3e

Cited: 20 times
EuropePMC logo PMID: 31964740

Abstract

Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is Acinetobacter baumannii, a Gram-negative opportunistic pathogen that exhibits the remarkable ability to resist antibiotics through multiple mechanisms. As bacterial ribosomes represent a target for multiple distinct classes of existing antimicrobial agents, we here use single-particle cryo-electron microscopy (cryo-EM) to elucidate five different structural states of the A. baumannii ribosome, including the 70S, 50S, and 30S forms. We also determined interparticle motions of the 70S ribosome in different tRNA bound states using three-dimensional (3D) variability analysis. Together, our structural data further our understanding of the ribosome from A. baumannii and other Gram-negative pathogens and will enable structure-based drug discovery to combat antibiotic-resistant bacterial infections.

Articles - 6v3d mentioned but not cited (1)

  1. Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development. Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW. mBio 11 e03117-19 (2020)


Reviews citing this publication (8)

  1. Structural Heterogeneities of the Ribosome: New Frontiers and Opportunities for Cryo-EM. Poitevin F, Kushner A, Li X, Dao Duc K. Molecules 25 E4262 (2020)
  2. The diversity of Shine-Dalgarno sequences sheds light on the evolution of translation initiation. Wen JD, Kuo ST, Chou HD. RNA Biol 18 1489-1500 (2021)
  3. New strategies and structural considerations in development of therapeutics for carbapenem-resistant Enterobacteriaceae. Brennan-Krohn T, Manetsch R, O'Doherty GA, Kirby JE. Transl Res 220 14-32 (2020)
  4. Advanced Methods for Studying Structure and Interactions of Macrolide Antibiotics. Jednačak T, Mikulandra I, Novak P. Int J Mol Sci 21 E7799 (2020)
  5. Cryo-EM as a tool to study bacterial efflux systems and the membrane proteome. Klenotic PA, Morgan CE, Yu EW. Fac Rev 10 24 (2021)
  6. In-cell Solid-State NMR Studies of Antimicrobial Peptides. Separovic F, Keizer DW, Sani MA. Front Med Technol 2 610203 (2020)
  7. Extraribosomal Functions of Bacterial Ribosomal Proteins-An Update, 2023. Aseev LV, Koledinskaya LS, Boni IV. Int J Mol Sci 25 2957 (2024)
  8. Imaging Infection Across Scales of Size: From Whole Animals to Single Molecules. Skaar EP. Annu Rev Microbiol 75 407-426 (2021)

Articles citing this publication (11)



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