6v3e › Citations

Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development.

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Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW
OpenAccess logo mBio 11 (2020)
Related entries: 6v39, 6v3a, 6v3b, 6v3d

Cited: 20 times
EuropePMC logo PMID: 31964740

Abstract

Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is Acinetobacter baumannii, a Gram-negative opportunistic pathogen that exhibits the remarkable ability to resist antibiotics through multiple mechanisms. As bacterial ribosomes represent a target for multiple distinct classes of existing antimicrobial agents, we here use single-particle cryo-electron microscopy (cryo-EM) to elucidate five different structural states of the A. baumannii ribosome, including the 70S, 50S, and 30S forms. We also determined interparticle motions of the 70S ribosome in different tRNA bound states using three-dimensional (3D) variability analysis. Together, our structural data further our understanding of the ribosome from A. baumannii and other Gram-negative pathogens and will enable structure-based drug discovery to combat antibiotic-resistant bacterial infections.

Articles - 6v3e mentioned but not cited (2)

  1. Annotating Macromolecular Complexes in the Protein Data Bank: Improving the FAIRness of Structure Data. Appasamy SD, Berrisford J, Gaborova R, Nair S, Anyango S, Grudinin S, Deshpande M, Armstrong D, Pidruchna I, Ellaway JIJ, Leines GD, Gupta D, Harrus D, Varadi M, Velankar S. Sci Data 10 853 (2023)
  2. Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development. Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW. mBio 11 (2020)


Reviews citing this publication (7)

  1. Advanced Methods for Studying Structure and Interactions of Macrolide Antibiotics. Jednačak T, Mikulandra I, Novak P. Int J Mol Sci 21 E7799 (2020)
  2. New strategies and structural considerations in development of therapeutics for carbapenem-resistant Enterobacteriaceae. Brennan-Krohn T, Manetsch R, O'Doherty GA, Kirby JE. Transl Res 220 14-32 (2020)
  3. Structural Heterogeneities of the Ribosome: New Frontiers and Opportunities for Cryo-EM. Poitevin F, Kushner A, Li X, Dao Duc K. Molecules 25 E4262 (2020)
  4. Cryo-EM as a tool to study bacterial efflux systems and the membrane proteome. Klenotic PA, Morgan CE, Yu EW. Fac Rev 10 24 (2021)
  5. Imaging Infection Across Scales of Size: From Whole Animals to Single Molecules. Skaar EP. Annu Rev Microbiol 75 407-426 (2021)
  6. In-cell Solid-State NMR Studies of Antimicrobial Peptides. Separovic F, Keizer DW, Sani MA. Front Med Technol 2 610203 (2020)
  7. The diversity of Shine-Dalgarno sequences sheds light on the evolution of translation initiation. Wen JD, Kuo ST, Chou HD. RNA Biol 18 1489-1500 (2021)

Articles citing this publication (11)

  1. Cryoelectron Microscopy Structures of AdeB Illuminate Mechanisms of Simultaneous Binding and Exporting of Substrates. Morgan CE, Glaza P, Leus IV, Trinh A, Su CC, Cui M, Zgurskaya HI, Yu EW. mBio 12 e03690-20 (2021)
  2. Cryo-EM Determination of Eravacycline-Bound Structures of the Ribosome and the Multidrug Efflux Pump AdeJ of Acinetobacter baumannii. Zhang Z, Morgan CE, Bonomo RA, Yu EW. mBio 12 e0103121 (2021)
  3. Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export. Moseng MA, Lyu M, Pipatpolkai T, Glaza P, Emerson CC, Stewart PL, Stansfeld PJ, Yu EW. mBio 12 e00452-21 (2021)
  4. Structure of the 70S Ribosome from the Human Pathogen Acinetobacter baumannii in Complex with Clinically Relevant Antibiotics. Nicholson D, Edwards TA, O'Neill AJ, Ranson NA. Structure 28 1087-1100.e3 (2020)
  5. Ratchet, swivel, tilt and roll: a complete description of subunit rotation in the ribosome. Hassan A, Byju S, Freitas FC, Roc C, Pender N, Nguyen K, Kimbrough EM, Mattingly JM, Gonzalez RL, de Oliveira RJ, Dunham CM, Whitford PC. Nucleic Acids Res 51 919-934 (2023)
  6. A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins. Su CC, Lyu M, Morgan CE, Bolla JR, Robinson CV, Yu EW. Nat Methods 18 69-75 (2021)
  7. An Analysis of the Novel Fluorocycline TP-6076 Bound to Both the Ribosome and Multidrug Efflux Pump AdeJ from Acinetobacter baumannii. Morgan CE, Zhang Z, Bonomo RA, Yu EW. mBio e0373221 (2022)
  8. Finding priority bacterial ribosomes for future structural and antimicrobial research based upon global RNA and protein sequence analysis. Cooper HB, Krause KL, Gardner PP. PeerJ 11 e14969 (2023)
  9. Maturation of 23S rRNA includes removal of helix H1 in many bacteria. Shatoff EA, Gemler BT, Bundschuh R, Fredrick K. RNA Biol 18 856-865 (2021)
  10. Persister control by leveraging dormancy associated reduction of antibiotic efflux. Roy S, Bahar AA, Gu H, Nangia S, Sauer K, Ren D. PLoS Pathog 17 e1010144 (2021)
  11. Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome. Morgan CE, Kang YS, Green AB, Smith KP, Dowgiallo MG, Miller BC, Chiaraviglio L, Truelson KA, Zulauf KE, Rodriguez S, Kang AD, Manetsch R, Yu EW, Kirby JE. PLoS Biol 21 e3002091 (2023)


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