› 6vz4

Electron Microscopy
3.9Å resolution

Cryo-EM structure of Sth1-Arp7-Arp9-Rtt102 bound to the nucleosome in ADP Beryllium Fluoride state

Released:
DOI: 10.2210/pdb6vz4/pdb
PDB entry 6vz4 coloured by chain, ensemble of 10 models, front view.
PDB entry 6vz4 coloured by chain, ensemble of 10 models, side view.
PDB entry 6vz4 coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
Structural insights into assembly and function of the RSC chromatin remodeling complex.
Baker RW, Reimer JM, Carman PJ, Turegun B, Arakawa T, Dominguez R, Leschziner AE
OpenAccess logo Nat Struct Mol Biol 28 71-80 (2021)
PMID: 33288924
Related structures: EMD-21484

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-120791 (preferred)
Entry contents:
8 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (10 distinct):
Histone H3 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.41 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92133 (Residues: 1-136; Coverage: 100%)
Gene names: H3l, Hist1h3f, h3, h3.2a, h3c8, h3c8.S, h3r, hist1h3g, hist1h3g.L, hist2h3, hist2h3c
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Histone H2B 1.1 Chains: D, H
Nuclear protein STH1/NPS1 Chain: K
Actin-related protein 7 Chain: L
Actin-like protein ARP9 Chain: M
Regulator of Ty1 transposition protein 102 Chain: N
DNA (185-MER) Chain: I
DNA (185-MER) Chain: J

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand BEF 1 x BEF
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-21484
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

Quick links

Citations

3 review citations

Structure and Function of Chromatin Remodelers.
Reyes et al. (2021)
2 more

1 mention without citation

Structural insights into assembly and function of the RSC chromatin remodeling complex.
Baker et al. (2021)