Structure analysis

Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1)

Electron Microscopy
3.47Å resolution
Source organism: Oryctolagus cuniculus
Assembly composition:
hetero 43-mer (preferred)
Entry contents: 41 distinct polypeptide molecules
2 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 43-mer
Accessible surface area: 597159.8 Å2
Buried surface area: 241274.44 Å2
Dissociation area: 2,489.45 Å2
Dissociation energy (ΔGdiss): 19.72 kcal/mol
Dissociation entropy (TΔSdiss): 15.04 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-244010

Macromolecules

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Chain: C
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SS70 (Residues: 1-264; Coverage: 100%)
Gene name: RPS3A
Pfam: Ribosomal S3Ae family
InterPro:

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Chain: H
Length: 249 amino acids
Theoretical weight: 28.75 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TM55 (Residues: 1-249; Coverage: 100%)
Gene name: RPS6
Pfam: Ribosomal protein S6e
InterPro:

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Chain: I
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SVB0 (Residues: 1-194; Coverage: 100%)
Gene name: RPS7
InterPro:

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Chain: J
Length: 208 amino acids
Theoretical weight: 24.26 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TJW1 (Residues: 1-208; Coverage: 100%)
Gene name: RPS8
Pfam: Ribosomal protein S8e
InterPro:

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Chain: P
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T1F0 (Residues: 1-151; Coverage: 100%)
Gene name: RPS14
Pfam: Ribosomal protein S11
InterPro:

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Chain: X
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TG89 (Residues: 1-130; Coverage: 100%)
Gene name: RPS15A
Pfam: Ribosomal protein S8
InterPro:

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Chain: Y
Length: 143 amino acids
Theoretical weight: 15.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SZ47 (Residues: 2-143; Coverage: 99%)
Gene name: RPS23
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: Z
Length: 134 amino acids
Theoretical weight: 15.55 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T3D8 (Residues: 1-131; Coverage: 100%)
Gene name: RPS24
Pfam: Ribosomal protein S24e
InterPro:

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Chain: b
Length: 115 amino acids
Theoretical weight: 13.02 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TFE8 (Residues: 1-115; Coverage: 100%)
Gene name: RPS26
Pfam: Ribosomal protein S26e
InterPro:

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Chain: c
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TZ76 (Residues: 1-84; Coverage: 100%)
Gene name: RPS27
Pfam: Ribosomal protein S27
InterPro:

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Chain: f
Length: 133 amino acids
Theoretical weight: 14.5 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T8A2 (Residues: 1-133; Coverage: 100%)
Gene name: FAU
Pfam:
InterPro:

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Chain: L
Length: 149 amino acids
Theoretical weight: 17.16 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TPV3 (Residues: 1-149; Coverage: 90%)
Pfam: Plectin/S10 domain
InterPro:

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Chain: N
Length: 132 amino acids
Theoretical weight: 14.54 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SFR8 (Residues: 1-132; Coverage: 100%)
Gene name: RPS12
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
InterPro:

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Chain: S
Length: 135 amino acids
Theoretical weight: 15.55 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TU13 (Residues: 1-135; Coverage: 100%)
Gene name: RPS17
Pfam: Ribosomal S17
InterPro:

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Chain: T
Length: 152 amino acids
Theoretical weight: 17.76 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TPG3 (Residues: 1-152; Coverage: 100%)
Gene name: RPS18
Pfam: Ribosomal protein S13/S18
InterPro:

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Chain: U
Length: 145 amino acids
Theoretical weight: 16.24 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TN62 (Residues: 1-145; Coverage: 100%)
Gene name: RPS19
Pfam: Ribosomal protein S19e
InterPro:

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Chain: a
Length: 125 amino acids
Theoretical weight: 13.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TDB3 (Residues: 1-124; Coverage: 100%)
Gene name: RPS25
Pfam: S25 ribosomal protein
InterPro:

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Chain: d
Length: 69 amino acids
Theoretical weight: 7.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TIB4 (Residues: 1-69; Coverage: 100%)
Gene name: RPS28
Pfam: Ribosomal protein S28e
InterPro:

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Chain: 1
Length: 1362 amino acids
Theoretical weight: 164.9 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SMZ5 (Residues: 1-1362; Coverage: 100%)
Gene names: EIF3A, EIF3S10
Pfam:
InterPro:

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Chain: 5
Length: 363 amino acids
Theoretical weight: 41.08 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1ST95 (Residues: 1-363; Coverage: 100%)
Gene names: EIF3H, EIF3S3
Pfam:
InterPro:

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Chain: 7
Length: 607 amino acids
Theoretical weight: 71 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SED9 (Residues: 1-607; Coverage: 100%)
Gene names: EIF3EIP, EIF3L, EIF3S6IP
Pfam: RNA polymerase I-associated factor PAF67
InterPro:

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Name: 18S Ribosomal RNA
Representative chains: A
Length: 1697 nucleotides
Theoretical weight: 547.73 KDa
Rfam: Eukaryotic small subunit ribosomal RNA

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Name: CrPV 5'-UTR IRES
Representative chains: 0
Length: 377 nucleotides
Theoretical weight: 121.09 KDa

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