› 6wi9

Electron Microscopy
4.3Å resolution

Human secretin receptor Gs complex

Released:
DOI: 10.2210/pdb6wi9/pdb
PDB entry 6wi9 coloured by chain, front view.
PDB entry 6wi9 coloured by chain, side view.
PDB entry 6wi9 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and dynamics of the active Gs-coupled human secretin receptor.
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
OpenAccess logo Nat Commun 11 4137 (2020)
PMID: 32811827
Related structures: EMD-21683

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209416 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Secretin Chain: P
Molecule details ›
Chain: P
Length: 27 amino acids
Theoretical weight: 3.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P09683 (Residues: 28-54; Coverage: 26%)
Gene name: SCT
Sequence domains: Peptide hormone
Secretin receptor Chain: R
Molecule details ›
Chain: R
Length: 453 amino acids
Theoretical weight: 51.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P47872 (Residues: 22-440; Coverage: 100%)
Gene name: SCTR
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-21683
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Capturing Peptide-GPCR Interactions and Their Dynamics.
Kaiser et al. (2020)
5 more

6 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
5 more