› 6wk5

X-ray diffraction
3.5Å resolution

Crystal structure of Gdx-Clo from Small Multidrug Resistance family of transporters

Released:
DOI: 10.2210/pdb6wk5/pdb
PDB entry 6wk5 coloured by chain, front view.
PDB entry 6wk5 coloured by chain, side view.
PDB entry 6wk5 coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis of promiscuity in small multidrug resistance transporters.
Kermani AA, Macdonald CB, Burata OE, Ben Koff B, Koide A, Denbaum E, Koide S, Stockbridge RB
OpenAccess logo Nat Commun 11 6064 (2020)
PMID: 33247110

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167719 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug resistance protein, SMR family Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 11.53 KDa
Source organism: Clostridiales bacterium oral taxon 876 str. F0540
Expression system: Escherichia coli
UniProt:
  • Canonical: U2EQ00 (Residues: 1-105; Coverage: 100%)
Gene name: HMPREF1982_00479
Sequence domains: Small Multidrug Resistance protein
L10 monobody Chains: C, D
Molecule details ›
Chains: C, D
Length: 91 amino acids
Theoretical weight: 9.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 141.8Å b: 51.093Å c: 108.433Å
α: 90° β: 93.08° γ: 90°
R-values:
R R work R free
0.253 0.252 0.286
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Advances in the Discovery of Efflux Pump Inhibitors as Novel Potentiators to Control Antimicrobial-Resistant Pathogens.
Zhang et al. (2023)
2 more

4 mentions without citation

The Role of the Membrane in Transporter Folding and Activity.
Ernst et al. (2021)
3 more