Assemblies
Assembly Name:
DNA-directed RNA polymerase holoenzyme complex, Sigma70 variant and DNA and unmapped proteins
Multimeric state:
hetero decamer
Accessible surface area:
185070.92 Å2
Buried surface area:
63288.6 Å2
Dissociation area:
1,731.29
Å2
Dissociation energy (ΔGdiss):
22.6
kcal/mol
Dissociation entropy (TΔSdiss):
15.99
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-120960
Macromolecules
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
Pfam:
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
- Canonical:
P0A7Z4 (Residues: 1-329; Coverage: 100%)
Pfam:
- RNA polymerase Rpb3/Rpb11 dimerisation domain
- RNA polymerase Rpb3/RpoA insert domain
- Bacterial RNA polymerase, alpha chain C terminal domain
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
Pfam:
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
- Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Pfam:
- RNA polymerase beta subunit
- RNA polymerase Rpb2, domain 2
- RNA polymerase Rpb2, domain 3
- RNA polymerase beta subunit external 1 domain
- RNA polymerase Rpb2, domain 6
- RNA polymerase Rpb2, domain 7
- DNA-directed RNA polymerase beta subunit, bacterial-type
- RNA polymerase, beta subunit, protrusion
- DNA-directed RNA polymerase, subunit 2
- RNA polymerase Rpb2, domain 2
- RNA polymerase Rpb2, domain 2 superfamily
- RNA polymerase Rpb2, domain 3
- DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily
- DNA-directed RNA polymerase, beta subunit, external 1 domain
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain
- RNA polymerase Rpb2, OB-fold
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily
- RNA polymerase, beta subunit, conserved site
- RNA polymerase Rpb2, domain 7
Chain: D
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
Pfam:
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
- Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Pfam:
- RNA polymerase Rpb1, domain 1
- RNA polymerase Rpb1, domain 2
- RNA polymerase Rpb1, domain 3
- RNA polymerase Rpb1, domain 4
- RNA polymerase Rpb1, domain 5
- RNA polymerase Rpb1, clamp domain superfamily
- DNA-directed RNA polymerase, subunit beta-prime, bacterial type
- RNA polymerase Rpb1, domain 1
- DNA-directed RNA polymerase, subunit beta-prime
- RNA polymerase, N-terminal
- RNA polymerase, alpha subunit
- RNA polymerase Rpb1, domain 3
- RNA polymerase Rpb1, domain 3 superfamily
- RNA polymerase Rpb1, funnel domain superfamily
- RNA polymerase Rpb1, domain 4
- RNA polymerase Rpb1, domain 5
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
Pfam: RNA polymerase Rpb6
InterPro:
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
- Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Pfam: RNA polymerase Rpb6
InterPro:
Chain: F
Length: 613 amino acids
Theoretical weight: 70.35 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
Pfam:
Length: 613 amino acids
Theoretical weight: 70.35 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
UniProt:
- Canonical: P00579 (Residues: 1-613; Coverage: 100%)
Pfam:
- Sigma-70 factor, region 1.1
- Sigma-70 factor, region 1.2
- Sigma-70, non-essential region
- Sigma-70 region 2
- Sigma-70 region 3
- Sigma-70, region 4
- Sigma-70 factor, region 1.1 superfamily
- RNA polymerase sigma factor 70, region 1.1
- RNA polymerase sigma factor RpoD
- RNA polymerase sigma-70 region 1.2
- RNA polymerase sigma factor, region 2
- RNA polymerase sigma factor 70, non-essential domain
- RNA polymerase sigma factor RpoD, C-terminal
- RNA polymerase sigma-70-like domain
- RNA polymerase sigma-70 region 2
- RNA polymerase sigma-70
- RNA polymerase sigma factor, region 3/4-like
- Winged helix-like DNA-binding domain superfamily
- RNA polymerase sigma-70 region 3
- RNA polymerase sigma-70 region 4
Chains: G, H
Length: 268 amino acids
Theoretical weight: 31.39 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
Length: 268 amino acids
Theoretical weight: 31.39 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli O157:H7
Name:
synthetic non-template strand DNA (54-MER)
Representative chains: N
Length: 54 nucleotides
Theoretical weight: 16.72 KDa
Representative chains: N
Length: 54 nucleotides
Theoretical weight: 16.72 KDa
Name:
synthetic template strand DNA (54-MER)
Representative chains: T
Length: 54 nucleotides
Theoretical weight: 16.63 KDa
Representative chains: T
Length: 54 nucleotides
Theoretical weight: 16.63 KDa
