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The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

McKenna MJ, Sim SI, Ordureau A, Wei L, Harper JW, Shao S, Park E
Science 369 (2020)
Related entries: 6xmp, 6xmq, 6xms, 6xmu

Cited: 56 times
EuropePMC logo PMID: 32973005

Abstract

Organelle identity depends on protein composition. How mistargeted proteins are selectively recognized and removed from organelles is incompletely understood. Here, we found that the orphan P5A-adenosine triphosphatase (ATPase) transporter ATP13A1 (Spf1 in yeast) directly interacted with the transmembrane segment (TM) of mitochondrial tail-anchored proteins. P5A-ATPase activity mediated the extraction of mistargeted proteins from the endoplasmic reticulum (ER). Cryo-electron microscopy structures of Saccharomyces cerevisiae Spf1 revealed a large, membrane-accessible substrate-binding pocket that alternately faced the ER lumen and cytosol and an endogenous substrate resembling an α-helical TM. Our results indicate that the P5A-ATPase could dislocate misinserted hydrophobic helices flanked by short basic segments from the ER. TM dislocation by the P5A-ATPase establishes an additional class of P-type ATPase substrates and may correct mistakes in protein targeting or topogenesis.

Articles - 6xmt mentioned but not cited (3)

  1. The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase. McKenna MJ, Sim SI, Ordureau A, Wei L, Harper JW, Shao S, Park E. Science 369 eabc5809 (2020)
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  3. Cryo-EM reveals mechanistic insights into lipid-facilitated polyamine export by human ATP13A2. Tomita A, Daiho T, Kusakizako T, Yamashita K, Ogasawara S, Murata T, Nishizawa T, Nureki O. Mol Cell 81 4799-4809.e5 (2021)


Reviews citing this publication (14)

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