› 6xot

Electron Microscopy
3.9Å resolution

CryoEM structure of human presequence protease in partial open state 2

Released:
DOI: 10.2210/pdb6xot/pdb
PDB entry 6xot coloured by chain, front view.
PDB entry 6xot coloured by chain, side view.
PDB entry 6xot coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition.
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
Nat Commun 13 1833 (2022)
PMID: 35383169
Related structures: EMD-22279

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Presequence protease, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 1014 amino acids
Theoretical weight: 114.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q5JRX3 (Residues: 33-1037; Coverage: 97%)
Gene names: KIAA1104, MP1, PITRM1, PREP
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-22279
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 citations in other articles

Insulin-Degrading Enzyme Interacts with Mitochondrial Ribosomes and Respiratory Chain Proteins.
Yilmaz et al. (2023)

1 mention without citation

Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition.
Liang et al. (2022)