› 6ygf

X-ray diffraction
1.7Å resolution

NADase from Aspergillus fumigatus with trapped reaction products

Released:
DOI: 10.2210/pdb6ygf/pdb
PDB entry 6ygf coloured by chain, front view.
PDB entry 6ygf coloured by chain, side view.
PDB entry 6ygf coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Discovery of fungal surface NADases predominantly present in pathogenic species.
Strømland Ø, Kallio JP, Pschibul A, Skoge RH, Harðardóttir HM, Sverkeli LJ, Heinekamp T, Kniemeyer O, Migaud M, Makarov MV, Gossmann TI, Brakhage AA, Ziegler M
OpenAccess logo Nat Commun 12 1631 (2021)
PMID: 33712585

Function and Biology Details

Reaction catalysed: 
NAD(+) + H(2)O = ADP-D-ribose + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Conidial surface nicotinamide adenine dinucleotide glycohydrolase nadA Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 27.68 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q4WL81 (Residues: 1-234; Coverage: 100%)
Gene names: AFUA_6G14470, nadA
Sequence domains: Tuberculosis necrotizing toxin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
5 bound ligands:
Ligand NAG 2 x NAG
Ligand GOL 3 x GOL
Ligand PO4 3 x PO4
Ligand NCA 1 x NCA
Ligand AR6 1 x AR6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P3221
Unit cell:
a: 63.815Å b: 63.815Å c: 257.723Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.201
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Emergence of the fungal immune system.
Daskalov A. (2023)
3 other articles

3 mentions without citation

Discovery of fungal surface NADases predominantly present in pathogenic species.
Strømland et al. (2021)
2 more