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Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.

Kater L, Mitterer V, Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E
Mol Cell 79 615-628.e5 (2020)
Related entries: 6yle, 6ylf, 6ylg, 6ylh, 6yly

Cited: 32 times
EuropePMC logo PMID: 32668200

Abstract

Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix12-Ipi32 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodeling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodeled by assembly factors, thereby ensuring failsafe assembly progression.

Reviews - 6ylx mentioned but not cited (1)

  1. RNA folding and functions of RNA helicases in ribosome biogenesis. Mitterer V, Pertschy B. RNA Biol 19 781-810 (2022)

Articles - 6ylx mentioned but not cited (6)



Reviews citing this publication (8)

  1. Ribosome biogenesis factors-from names to functions. Dörner K, Ruggeri C, Zemp I, Kutay U. EMBO J 42 e112699 (2023)
  2. AAA+ ATPases: structural insertions under the magnifying glass. Jessop M, Felix J, Gutsche I. Curr Opin Struct Biol 66 119-128 (2021)
  3. AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors. Zhang G, Li S, Cheng KW, Chou TF. Eur J Med Chem 219 113446 (2021)
  4. Ubiquitin and Ubiquitin-Like Proteins and Domains in Ribosome Production and Function: Chance or Necessity? Martín-Villanueva S, Gutiérrez G, Kressler D, de la Cruz J. Int J Mol Sci 22 4359 (2021)
  5. Quality control ensures fidelity in ribosome assembly and cellular health. Parker MD, Karbstein K. J Cell Biol 222 e202209115 (2023)
  6. Structural overview of macromolecular machines involved in ribosome biogenesis. Frazier MN, Pillon MC, Kocaman S, Gordon J, Stanley RE. Curr Opin Struct Biol 67 51-60 (2021)
  7. Diversity and Similarity of Termination and Ribosome Rescue in Bacterial, Mitochondrial, and Cytoplasmic Translation. Korostelev AA. Biochemistry (Mosc) 86 1107-1121 (2021)
  8. Putting It All Together: The Roles of Ribosomal Proteins in Nucleolar Stages of 60S Ribosomal Assembly in the Yeast Saccharomyces cerevisiae. Ayers TN, Woolford JL. Biomolecules 14 975 (2024)

Articles citing this publication (17)



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