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Type III ATP synthase is a symmetry-deviated dimer that induces membrane curvature through tetramerization.

OpenAccess logo Nat Commun 11 5342 (2020)
Related entries: 6ynv, 6ynw, 6ynx, 6yny, 6ynz

Cited: 19 times
EuropePMC logo PMID: 33093501

Abstract

Mitochondrial ATP synthases form functional homodimers to induce cristae curvature that is a universal property of mitochondria. To expand on the understanding of this fundamental phenomenon, we characterized the unique type III mitochondrial ATP synthase in its dimeric and tetrameric form. The cryo-EM structure of a ciliate ATP synthase dimer reveals an unusual U-shaped assembly of 81 proteins, including a substoichiometrically bound ATPTT2, 40 lipids, and co-factors NAD and CoQ. A single copy of subunit ATPTT2 functions as a membrane anchor for the dimeric inhibitor IF1. Type III specific linker proteins stably tie the ATP synthase monomers in parallel to each other. The intricate dimer architecture is scaffolded by an extended subunit-a that provides a template for both intra- and inter-dimer interactions. The latter results in the formation of tetramer assemblies, the membrane part of which we determined to 3.1 Å resolution. The structure of the type III ATP synthase tetramer and its associated lipids suggests that it is the intact unit propagating the membrane curvature.

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  2. The mystery of massive mitochondrial complexes: the apicomplexan respiratory chain. Maclean AE, Hayward JA, Huet D, van Dooren GG, Sheiner L. Trends Parasitol 38 1041-1052 (2022)
  3. CryoEM Reveals the Complexity and Diversity of ATP Synthases. Courbon GM, Rubinstein JL. Front Microbiol 13 864006 (2022)
  4. Identity, structure, and function of the mitochondrial permeability transition pore: controversies, consensus, recent advances, and future directions. Bernardi P, Gerle C, Halestrap AP, Jonas EA, Karch J, Mnatsakanyan N, Pavlov E, Sheu SS, Soukas AA. Cell Death Differ 30 1869-1885 (2023)
  5. The ATPase Inhibitory Factor 1 is a Tissue-Specific Physiological Regulator of the Structure and Function of Mitochondrial ATP Synthase: A Closer Look Into Neuronal Function. Domínguez-Zorita S, Romero-Carramiñana I, Cuezva JM, Esparza-Moltó PB. Front Physiol 13 868820 (2022)

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