› 6yvd

Electron Microscopy
7.6Å resolution

Head segment of the S.cerevisiae condensin holocomplex in presence of ATP

Released:
DOI: 10.2210/pdb6yvd/pdb
PDB entry 6yvd coloured by chain, front view.
PDB entry 6yvd coloured by chain, side view.
PDB entry 6yvd coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism.
Lee BG, Merkel F, Allegretti M, Hassler M, Cawood C, Lecomte L, O'Reilly FJ, Sinn LR, Gutierrez-Escribano P, Kschonsak M, Bravo S, Nakane T, Rappsilber J, Aragon L, Beck M, Löwe J, Haering CH
Nat Struct Mol Biol 27 743-751 (2020)
PMID: 32661420
Related structures: EMD-10944

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-153564 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Condensin complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 811 amino acids
Theoretical weight: 92.73 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38170 (Residues: 1-754; Coverage: 100%)
Gene names: BRN1, YBL0830, YBL097W
Sequence domains: Condensin complex subunit 2
Condensin complex subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 1035 amino acids
Theoretical weight: 117.98 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q06680 (Residues: 1-1035; Coverage: 100%)
Gene names: YCG1, YCS5, YDR325W
Sequence domains: Nuclear condensing complex subunits, C-term domain
Structural maintenance of chromosomes protein 2 Chain: C
Molecule details ›
Chain: C
Length: 1170 amino acids
Theoretical weight: 134.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38989 (Residues: 1-1170; Coverage: 100%)
Gene names: SMC2, YFR031C
Sequence domains:
Structural maintenance of chromosomes protein 4 Chain: D
Molecule details ›
Chain: D
Length: 1478 amino acids
Theoretical weight: 168.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12267 (Residues: 1-1418; Coverage: 100%)
Gene names: L9449.5, SMC4, YLR086W
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.6Å
Relevant EMDB volumes: EMD-10944
Expression system: Saccharomyces cerevisiae

Quick links

Citations

8 review citations

Genome folding through loop extrusion by SMC complexes.
Davidson et al. (2021)
7 more

1 mention without citation

Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism.
Lee et al. (2020)