6yym

X-ray diffraction
2.63Å resolution

Structure of S. pombe Mei2 RRM3 domain bound to RNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-140450 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Meiosis protein mei2 Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.6 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P08965 (Residues: 579-750; Coverage: 23%)
Gene names: SPAC27D7.03c, mei2
Sequence domains: RNA recognition motif 2
RNA (5'-R(*GP*CP*UP*UP*UP*UP*UP*GP*UP*UP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.74 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I222
Unit cell:
a: 75.27Å b: 81.48Å c: 81.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.223 0.298
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided