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Structure of S. pombe Mei2 RRM3 domain bound to RNA

X-ray diffraction
2.63Å resolution
PDB entry 6yym coloured by chain, front view.
PDB entry 6yym coloured by chain, side view.
PDB entry 6yym coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Meiosis protein mei2 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 9027.3 Å2
Buried surface area: 2416.1 Å2
Dissociation area: 1,208.05 Å2
Dissociation energy (ΔGdiss): 17.45 kcal/mol
Dissociation entropy (TΔSdiss): 10.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140450

Macromolecules

Meiosis protein mei2
Chain: A
Length: 182 amino acids
Theoretical weight: 20.6 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P08965 (Residues: 579-750; Coverage: 23%)
Gene names: SPAC27D7.03c, mei2
Pfam: RNA recognition motif 2
InterPro:
Meiosis protein mei2 in PDB entry 6yym, assembly 1, front view.
Meiosis protein mei2 in PDB entry 6yym, assembly 1, side view.
Meiosis protein mei2 in PDB entry 6yym, assembly 1, top view.
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RNA chain B
Name: RNA (5'-R(*GP*CP*UP*UP*UP*UP*UP*GP*UP*UP*CP*G)-3')
Representative chains: B
Source organism: Schizosaccharomyces pombe [4896]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.74 KDa
RNA (5'-R(*GP*CP*UP*UP*UP*UP*UP*GP*UP*UP*CP*G)-3') in PDB entry 6yym, assembly 1, front view.
RNA (5'-R(*GP*CP*UP*UP*UP*UP*UP*GP*UP*UP*CP*G)-3') in PDB entry 6yym, assembly 1, side view.
RNA (5'-R(*GP*CP*UP*UP*UP*UP*UP*GP*UP*UP*CP*G)-3') in PDB entry 6yym, assembly 1, top view.
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