6zfn

X-ray diffraction
1.1Å resolution

Structure of an inactive E404Q variant of the catalytic domain of human endo-alpha-mannosidase MANEA in complex with 1-methyl alpha-1,2-mannobiose

Released:

Function and Biology Details

Reaction catalysed:
GlcMan(9)GlcNAc(2)-[protein] + H(2)O = Man(8)GlcNAc(2)-[protein] (isomer 8A(1,2,3)B(1,2)) + alpha-D-glucosyl-(1->3)-alpha-D-mannopyranose
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-178277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoprotein endo-alpha-1,2-mannosidase Chain: AAA
Molecule details ›
Chain: AAA
Length: 382 amino acids
Theoretical weight: 44.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SRI9 (Residues: 98-462; Coverage: 79%)
Gene name: MANEA
Sequence domains: Glycosyl hydrolase family 99

Ligands and Environments

Carbohydrate polymer : NEW Components: MMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 42.752Å b: 81.68Å c: 52.994Å
α: 90° β: 92.934° γ: 90°
R-values:
R R work R free
0.107 0.106 0.128
Expression system: Escherichia coli BL21(DE3)