6ztl Citations

Structural basis of transcription-translation coupling and collision in bacteria.

Science 369 1355-1359 (2020)
Related entries: 6ztj, 6ztm, 6ztn, 6zto, 6ztp, 6zu1

Cited: 49 times
EuropePMC logo PMID: 32820062

Abstract

Prokaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The lead ribosome potentially contacts RNA polymerase (RNAP) and forms a supramolecular complex known as the expressome. The basis of expressome assembly and its consequences for transcription and translation are poorly understood. Here, we present a series of structures representing uncoupled, coupled, and collided expressome states determined by cryo-electron microscopy. A bridge between the ribosome and RNAP can be formed by the transcription factor NusG, which stabilizes an otherwise-variable interaction interface. Shortening of the intervening mRNA causes a substantial rearrangement that aligns the ribosome entrance channel to the RNAP exit channel. In this collided complex, NusG linkage is no longer possible. These structures reveal mechanisms of coordination between transcription and translation and provide a framework for future study.

Reviews - 6ztl mentioned but not cited (1)

  1. Macromolecular assemblies supporting transcription-translation coupling. Webster MW, Weixlbaumer A. Transcription 12 103-125 (2021)

Articles - 6ztl mentioned but not cited (1)



Reviews citing this publication (12)

  1. Translational Control by Ribosome Pausing in Bacteria: How a Non-uniform Pace of Translation Affects Protein Production and Folding. Samatova E, Daberger J, Liutkute M, Rodnina MV. Front Microbiol 11 619430 (2020)
  2. Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises. Irastortza-Olaziregi M, Amster-Choder O. Front Microbiol 11 624830 (2020)
  3. NusG, an Ancient Yet Rapidly Evolving Transcription Factor. Wang B, Artsimovitch I. Front Microbiol 11 619618 (2020)
  4. Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future. Burley SK, Berman HM, Chiu W, Dai W, Flatt JW, Hudson BP, Kaelber JT, Khare SD, Kulczyk AW, Lawson CL, Pintilie GD, Sali A, Vallat B, Westbrook JD, Young JY, Zardecki C. Biophys Rev 14 1281-1301 (2022)
  5. The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches. Gerovac M, Vogel J, Smirnov A. Front Mol Biosci 8 661448 (2021)
  6. How structural biology transformed studies of transcription regulation. Wolberger C. J Biol Chem 296 100741 (2021)
  7. Composition of Transcription Machinery and Its Crosstalk with Nucleoid-Associated Proteins and Global Transcription Factors. Muskhelishvili G, Sobetzko P, Mehandziska S, Travers A. Biomolecules 11 924 (2021)
  8. Bacterial transcription during growth arrest. Bergkessel M. Transcription 12 232-249 (2021)
  9. Structural advances in transcription elongation. Mohamed AA, Vazquez Nunez R, Vos SM. Curr Opin Struct Biol 75 102422 (2022)
  10. Exploring the Structural Variability of Dynamic Biological Complexes by Single-Particle Cryo-Electron Microscopy. DiIorio MC, Kulczyk AW. Micromachines (Basel) 14 118 (2022)
  11. The molecular basis of translation initiation and its regulation in eukaryotes. Brito Querido J, Díaz-López I, Ramakrishnan V. Nat Rev Mol Cell Biol 25 168-186 (2024)
  12. Riboswitches as therapeutic targets: promise of a new era of antibiotics. Ellinger E, Chauvier A, Romero RA, Liu Y, Ray S, Walter NG. Expert Opin Ther Targets 27 433-445 (2023)

Articles citing this publication (35)