7bhf

X-ray diffraction
2Å resolution

DARPin_D5/Her3 domain 4 complex, orthorhombic crystals

Released:
Primary publication:
Crystal structures of HER3 extracellular domain 4 in complex with the designed ankyrin-repeat protein D5.
OpenAccess logo Acta Crystallogr F Struct Biol Commun 77 192-201 (2021)
PMID: 34196609

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-539961 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DARPin_D5 Chains: A, C
Molecule details ›
Chains: A, C
Length: 138 amino acids
Theoretical weight: 14.91 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Receptor tyrosine-protein kinase erbB-3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 150 amino acids
Theoretical weight: 16.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 62.258Å b: 64.951Å c: 144.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.243
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda