7old

Electron Microscopy
3Å resolution

Thermophilic eukaryotic 80S ribosome at pe/E (TI)-POST state

Released:

Function and Biology Details

Reaction catalysed:
Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 85-mer (preferred)
PDBe Complex ID:
PDB-CPX-123880 (preferred)
Entry contents:
79 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (84 distinct):
Putative guanine nucleotide-binding protein Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 35.15 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Hyaluronan/mRNA-binding protein domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 302 amino acids
Theoretical weight: 32.67 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Elongation factor 2 Chain: C
Large ribosomal subunit protein uL2 C-terminal domain-containing protein Chain: LA
60S ribosomal protein L3-like protein Chain: LB
Large ribosomal subunit protein uL4 C-terminal domain-containing protein Chain: LC
Large ribosomal subunit protein uL18 Chain: LD
60S ribosomal protein L6 Chain: LE
60S ribosomal protein l7-like protein Chain: LF
60S ribosomal protein L8 Chain: LG
Large ribosomal subunit protein uL6 alpha-beta domain-containing protein Chain: LH
60S ribosomal protein L10-like protein Chain: LI
Large ribosomal subunit protein uL5 Chain: LJ
60S ribosomal protein L12-like protein Chain: LK
60S ribosomal protein L13 Chain: LL
Large ribosomal subunit protein eL14 domain-containing protein Chain: LM
Ribosomal protein L15 Chain: LN
60S ribosomal protein L16-like protein Chain: LO
60S ribosomal protein l17-like protein Chain: LP
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: LQ
Ribosomal protein L19 Chain: LR
60S ribosomal protein L20 Chain: LS
60S ribosomal protein l21-like protein Chain: LT
60S ribosomal protein L22-like protein Chain: LU
60S ribosomal protein l23-like protein Chain: LV
Large ribosomal subunit protein eL24-related N-terminal domain-containing protein Chain: LW
Large ribosomal subunit protein uL23 N-terminal domain-containing protein Chain: LX
KOW domain-containing protein Chain: LY
60S ribosomal protein L27 Chain: LZ
60S ribosomal protein L28-like protein Chain: La
60S ribosomal protein L29 Chain: Lb
Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein Chain: Lc
Putative 60S ribosomal protein Chain: Ld
60S ribosomal protein L32-like protein Chain: Le
60S ribosomal protein l33-like protein Chain: Lf
Ribosomal protein l34-like protein Chain: Lg
dolichyl-diphosphooligosaccharide--protein glycotransferase Chain: Lh
60S ribosomal protein L36 Chain: Li
Ribosomal protein L37 Chain: Lj
60S ribosomal protein L38-like protein Chain: Lk
Large ribosomal subunit protein eL39 Chain: Ll
Ubiquitin-like domain-containing protein Chain: Lm
Small ribosomal subunit protein eS32A Chains: Ln, Lr
60S ribosomal protein L44-like protein Chain: Lo
60S ribosomal protein L43-like protein Chain: Lp
Ribosomal eL28/Mak16 domain-containing protein Chain: Lq
60S acidic ribosomal protein P0 Chain: Ls
Small ribosomal subunit protein uS2 Chain: SA
Small ribosomal subunit protein eS1 Chain: SB
Small ribosomal subunit protein uS5 Chain: SC
40S ribosomal protein S3 Chain: SD
40S ribosomal protein S4 Chain: SE
Small ribosomal subunit protein uS7 domain-containing protein Chain: SF
40S ribosomal protein S6 Chain: SG
40S ribosomal protein S7 Chain: SH
40S ribosomal protein S8 Chain: SI
40S ribosomal protein s9-like protein Chain: SJ
Plectin/eS10 N-terminal domain-containing protein Chain: SK
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: SL
40S ribosomal protein S12 Chain: SM
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: SN
40S ribosomal protein S14-like protein Chain: SO
40S ribosomal protein s15-like protein Chain: SP
40S ribosomal protein S16-like protein Chain: SQ
40S ribosomal protein S17-like protein Chain: SR
Putative ribosomal protein Chain: SS
40S ribosomal protein S19-like protein Chain: ST
Small ribosomal subunit protein uS10 domain-containing protein Chain: SU
40S ribosomal protein S21-like protein Chain: SV
40S ribosomal protein S22-like protein Chain: SW
40S ribosomal protein s23-like protein Chain: SX
Small ribosomal subunit protein eS24 Chain: SY
40S ribosomal protein S25 Chain: SZ
40S ribosomal protein S26 Chain: Sa
Ribosomal protein s27-like protein Chain: Sb
40S ribosomal protein S28-like protein Chain: Sc
40S ribosomal protein S29 Chain: Sd
40S ribosomal protein S30 Chain: Se
Ubiquitin-like domain-containing protein Chain: Sf
26S rRNA Chain: 1
18S Ribosomal RNA Chain: 2
5S ribosomal RNA Chain: 3
5.8S ribosomal RNA Chain: 4
pe/E tRNA Chain: 5

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-12977