› 7wqz

Electron Microscopy
3.7Å resolution

Structure of Active-mutEP

Released:
DOI: 10.2210/pdb7wqz/pdb
PDB entry 7wqz coloured by chain, front view.
PDB entry 7wqz coloured by chain, side view.
PDB entry 7wqz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures reveal the activation and substrate recognition mechanism of human enteropeptidase.
Yang X, Ding Z, Peng L, Song Q, Zhang D, Cui F, Xia C, Li K, Yin H, Li S, Li Z, Huang H
OpenAccess logo Nat Commun 13 6955 (2022)
PMID: 36376282
Related structures: EMD-32716

Function and Biology Details

Reaction catalysed: 
Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161344 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Enteropeptidase non-catalytic heavy chain Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P98073 (Residues: 524-784; Coverage: 26%)
Gene names: ENTK, PRSS7, TMPRSS15
Sequence domains:
Enteropeptidase catalytic light chain Chain: B
Molecule details ›
Chain: B
Length: 235 amino acids
Theoretical weight: 26.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P98073 (Residues: 785-1019; Coverage: 23%)
Gene names: ENTK, PRSS7, TMPRSS15
Sequence domains: Trypsin

Ligands and Environments

1 bound ligand:
Ligand NAG 9 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-32716
Expression system: Homo sapiens

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