› 7a8p

Electron Microscopy
3.5Å resolution

Structure of human mitochondrial RNA polymerase in complex with IMT inhibitor.

Released:
DOI: 10.2210/pdb7a8p/pdb
PDB entry 7a8p coloured by chain, front view.
PDB entry 7a8p coloured by chain, side view.
PDB entry 7a8p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Small-molecule inhibitors of human mitochondrial DNA transcription.
Bonekamp NA, Peter B, Hillen HS, Felser A, Bergbrede T, Choidas A, Horn M, Unger A, Di Lucrezia R, Atanassov I, Li X, Koch U, Menninger S, Boros J, Habenberger P, Giavalisco P, Cramer P, Denzel MS, Nussbaumer P, Klebl B, Falkenberg M, Gustafsson CM, Larsson NG
Nature 588 712-716 (2020)
PMID: 33328633
Related structures: EMD-11679

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 1143 amino acids
Theoretical weight: 129.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00411 (Residues: 105-1230; Coverage: 92%)
Gene name: POLRMT
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand R4Q 1 x R4Q
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-11679
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Cancer metabolism: looking forward.
Martínez-Reyes et al. (2021)
28 more

2 mentions without citation

Targeting Mitochondrial Metabolism and RNA Polymerase POLRMT to Overcome Multidrug Resistance in Cancer.
Yu et al. (2021)
1 more