› 7ac7

Electron Microscopy
3.08Å resolution

Structure of accomodated trans-translation complex on E. Coli stalled ribosome.

Released:
DOI: 10.2210/pdb7ac7/pdb
PDB entry 7ac7 coloured by chain, front view.
PDB entry 7ac7 coloured by chain, side view.
PDB entry 7ac7 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of tmRNA and SmpB as they transit through the ribosome.
Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R
OpenAccess logo Nat Commun 12 4909 (2021)
PMID: 34389707
Related structures: EMD-11713

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 58-mer (preferred)
PDBe Complex ID:
PDB-CPX-135575 (preferred)
Entry contents:
52 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (58 distinct):
SsrA-binding protein Chain: 5
Molecule details ›
Chain: 5
Length: 147 amino acids
Theoretical weight: 16.93 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
Large ribosomal subunit protein bL27 Chain: A
Molecule details ›
Chain: A
Length: 84 amino acids
Theoretical weight: 9.02 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL2 Chain: B
Molecule details ›
Chain: B
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL3 Chain: C
Molecule details ›
Chain: C
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL4 Chain: D
Molecule details ›
Chain: D
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL5 Chain: E
Molecule details ›
Chain: E
Length: 177 amino acids
Theoretical weight: 20.07 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL6 Chain: F
Molecule details ›
Chain: F
Length: 175 amino acids
Theoretical weight: 18.67 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL9 Chain: G
Molecule details ›
Chain: G
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL10 Chain: H
Molecule details ›
Chain: H
Length: 130 amino acids
Theoretical weight: 13.98 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL13 Chain: J
Molecule details ›
Chain: J
Length: 141 amino acids
Theoretical weight: 15.92 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL14 Chain: K
Molecule details ›
Chain: K
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL15 Chain: L
Molecule details ›
Chain: L
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL16 Chain: M
Molecule details ›
Chain: M
Length: 136 amino acids
Theoretical weight: 15.33 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL17 Chain: N
Molecule details ›
Chain: N
Length: 119 amino acids
Theoretical weight: 13.59 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL18 Chain: O
Molecule details ›
Chain: O
Length: 115 amino acids
Theoretical weight: 12.55 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL19 Chain: P
Molecule details ›
Chain: P
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL20 Chain: Q
Molecule details ›
Chain: Q
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL21 Chain: R
Molecule details ›
Chain: R
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL22 Chain: S
Molecule details ›
Chain: S
Length: 109 amino acids
Theoretical weight: 12.12 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL23 Chain: T
Molecule details ›
Chain: T
Length: 94 amino acids
Theoretical weight: 10.66 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL24 Chain: U
Molecule details ›
Chain: U
Length: 103 amino acids
Theoretical weight: 11.21 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL25 Chain: V
Molecule details ›
Chain: V
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli K-12
Nascent peptide Chain: W
Molecule details ›
Chain: W
Length: 2 amino acids
Theoretical weight: 236 Da
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL28 Chain: X
Molecule details ›
Chain: X
Length: 77 amino acids
Theoretical weight: 8.9 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL29 Chain: Y
Molecule details ›
Chain: Y
Length: 58 amino acids
Theoretical weight: 6.83 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein uL30 Chain: Z
Molecule details ›
Chain: Z
Length: 56 amino acids
Theoretical weight: 6.22 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL31 Chain: a
Molecule details ›
Chain: a
Length: 66 amino acids
Theoretical weight: 7.52 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL32 Chain: b
Molecule details ›
Chain: b
Length: 54 amino acids
Theoretical weight: 6.13 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL33 Chain: c
Molecule details ›
Chain: c
Length: 52 amino acids
Theoretical weight: 6.06 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL34 Chain: d
Molecule details ›
Chain: d
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL35 Chain: e
Molecule details ›
Chain: e
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Escherichia coli K-12
Large ribosomal subunit protein bL36A Chain: f
Molecule details ›
Chain: f
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS2 Chain: g
Molecule details ›
Chain: g
Length: 225 amino acids
Theoretical weight: 25.07 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS3 Chain: h
Molecule details ›
Chain: h
Length: 208 amino acids
Theoretical weight: 23.25 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS4 Chain: i
Molecule details ›
Chain: i
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS5 Chain: j
Molecule details ›
Chain: j
Length: 156 amino acids
Theoretical weight: 16.48 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein bS6, non-modified isoform Chain: k
Molecule details ›
Chain: k
Length: 104 amino acids
Theoretical weight: 12.13 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS7 Chain: l
Molecule details ›
Chain: l
Length: 151 amino acids
Theoretical weight: 16.86 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS8 Chain: m
Molecule details ›
Chain: m
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS9 Chain: n
Molecule details ›
Chain: n
Length: 126 amino acids
Theoretical weight: 14.4 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS10 Chain: o
Molecule details ›
Chain: o
Length: 99 amino acids
Theoretical weight: 11.25 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS11 Chain: p
Molecule details ›
Chain: p
Length: 117 amino acids
Theoretical weight: 12.49 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS12 Chain: q
Molecule details ›
Chain: q
Length: 123 amino acids
Theoretical weight: 13.68 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS13 Chain: r
Molecule details ›
Chain: r
Length: 115 amino acids
Theoretical weight: 12.74 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS14 Chain: s
Molecule details ›
Chain: s
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS15 Chain: t
Molecule details ›
Chain: t
Length: 87 amino acids
Theoretical weight: 10.07 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein bS16 Chain: u
Molecule details ›
Chain: u
Length: 81 amino acids
Theoretical weight: 9.14 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS17 Chain: v
Molecule details ›
Chain: v
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein bS18 Chain: w
Molecule details ›
Chain: w
Length: 65 amino acids
Theoretical weight: 7.61 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein uS19 Chain: x
Molecule details ›
Chain: x
Length: 82 amino acids
Theoretical weight: 9.35 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein bS20 Chain: y
Molecule details ›
Chain: y
Length: 85 amino acids
Theoretical weight: 9.51 KDa
Source organism: Escherichia coli K-12
Small ribosomal subunit protein bS21 Chain: z
Molecule details ›
Chain: z
Length: 70 amino acids
Theoretical weight: 8.39 KDa
Source organism: Escherichia coli K-12
23S ribosomal RNA Chain: 1
Molecule details ›
Chain: 1
Length: 2903 nucleotides
Theoretical weight: 941.53 KDa
Source organism: Escherichia coli K-12
Sequence domains: Bacterial large subunit ribosomal RNA
16S ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 1540 nucleotides
Theoretical weight: 499.24 KDa
Source organism: Escherichia coli K-12
Sequence domains: Bacterial small subunit ribosomal RNA
5S ribosomal RNA Chain: 3
Molecule details ›
Chain: 3
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Source organism: Escherichia coli K-12
Sequence domains: 5S ribosomal RNA
transfer-messenger RNA (tmRNA) Chain: 4
Molecule details ›
Chain: 4
Length: 363 nucleotides
Theoretical weight: 117.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: transfer-messenger RNA
tRNA-Phe Chain: 7
Molecule details ›
Chain: 7
Length: 76 nucleotides
Theoretical weight: 24.75 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: tRNA
Messenger RNA Chain: 8
Molecule details ›
Chain: 8
Length: 15 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 441 x MG
Ligand ZN 2 x ZN
21 modified residues:
Ligand 1MG 1 x 1MG
Ligand PSU 14 x PSU
Ligand 5MU 4 x 5MU
Ligand 6MZ 2 x 6MZ
Ligand 2MG 5 x 2MG
Ligand 3TD 1 x 3TD
Ligand 5MC 3 x 5MC
Ligand G7M 3 x G7M
Ligand OMG 1 x OMG
Ligand OMC 1 x OMC
Ligand 2MA 1 x 2MA
Ligand OMU 1 x OMU
Ligand 4OC 1 x 4OC
Ligand UR3 1 x UR3
Ligand MA6 2 x MA6
Ligand 4SU 1 x 4SU
Ligand H2U 2 x H2U
Ligand MIA 1 x MIA
Ligand 3AU 1 x 3AU
Ligand 4D4 1 x 4D4
Ligand 0TD 1 x 0TD

Experiments and Validation Details

Entry percentile scores
Resolution: 3.08Å
Relevant EMDB volumes: EMD-11713
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Trans-Translation Is an Appealing Target for the Development of New Antimicrobial Compounds.
Campos-Silva et al. (2021)
6 other articles

2 mentions without citation

Bacterial Ribosome Rescue Systems.
Kurita et al. (2022)
1 more