› 7akz

X-ray diffraction
3.2Å resolution

Deciphering the role of the channel constrictions in the opening mechanism of MexAB-OprM efflux pump from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb7akz/pdb
PDB entry 7akz coloured by chain, front view.
PDB entry 7akz coloured by chain, side view.
PDB entry 7akz coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Ntsogo Enguene VY, Monlezun L, Ma M, Garnier C, Lascombe MB, Salem M, Guenard S, Plesiat P, Llanes C, Phan G, Broutin I

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein OprM Chains: A, B
Molecule details ›
Chains: A, B
Length: 474 amino acids
Theoretical weight: 51.49 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51487 (Residues: 18-485; Coverage: 100%)
Gene names: PA0427, oprK, oprM
Sequence domains: Outer membrane efflux protein

Ligands and Environments

2 bound ligands:
Ligand PLM 1 x PLM
Ligand BOG 3 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: R32
Unit cell:
a: 86.059Å b: 86.059Å c: 1,049.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.273 0.27 0.318
Expression system: Escherichia coli BL21(DE3)

Quick links