Structure analysis

Cryo-EM structure of Arabidopsis thaliana Complex-I (peripheral arm)

Electron Microscopy
2.91Å resolution
Source organism: Arabidopsis thaliana
Assembly composition:
hetero heptadecamer (preferred)
Entry contents: 17 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero heptadecamer
Accessible surface area: 117558.8 Å2
Buried surface area: 63904.23 Å2
Dissociation area: 1,186.81 Å2
Dissociation energy (ΔGdiss): -5.38 kcal/mol
Dissociation entropy (TΔSdiss): 19.6 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-127641

Macromolecules

Chain: B
Length: 218 amino acids
Theoretical weight: 24.07 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q42577 (Residues: 1-218; Coverage: 100%)
Gene names: At5g11770, T22P22_160
Pfam: NADH ubiquinone oxidoreductase, 20 Kd subunit
InterPro:

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Chain: E
Length: 255 amino acids
Theoretical weight: 28.42 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: O22769 (Residues: 1-255; Coverage: 100%)
Gene names: At4g02580, T10P11.14
Pfam: Thioredoxin-like [2Fe-2S] ferredoxin
InterPro:

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Chain: I
Length: 222 amino acids
Theoretical weight: 25.54 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q42599 (Residues: 1-222; Coverage: 100%)
Gene names: At1g79010, YUP8H12R.37, YUP8H12R_21
Pfam: 4Fe-4S dicluster domain
InterPro:

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Chain: P
Length: 402 amino acids
Theoretical weight: 43.99 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9SK66 (Residues: 1-402; Coverage: 100%)
Gene names: At2g20360, F11A3.9
Pfam: NAD dependent epimerase/dehydratase family
InterPro:

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Chain: Q
Length: 154 amino acids
Theoretical weight: 17.16 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9FJW4 (Residues: 1-154; Coverage: 100%)
Gene names: At5g67590, FRO1, K9I9.16
Pfam: NADH dehydrogenase ubiquinone Fe-S protein 4
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Chain: R
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9M9M6 (Residues: 1-110; Coverage: 100%)
Gene names: At3g03070, T17B22_24
Pfam: Zinc-finger domain
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Chain: S
Length: 97 amino acids
Theoretical weight: 10.87 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9FIJ2 (Residues: 1-97; Coverage: 100%)
Gene names: At5g47890, MCA23_23
Pfam: Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain
InterPro:

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Chain: U
Length: 126 amino acids
Theoretical weight: 14.18 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: O80800 (Residues: 1-126; Coverage: 100%)
Gene names: At1g65290, MTACP2, T8F5.6
Pfam: Phosphopantetheine attachment site
InterPro:

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Chain: V
Length: 169 amino acids
Theoretical weight: 19.2 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9FLX7 (Residues: 1-169; Coverage: 100%)
Gene names: At5g52840, MXC20.6
Pfam: ETC complex I subunit conserved region
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

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Chain: W
Length: 133 amino acids
Theoretical weight: 15.1 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9LHI0 (Residues: 1-133; Coverage: 100%)
Gene names: At3g12260, F28J15.12, MQC3.9
InterPro:

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Chain: Z
Length: 143 amino acids
Theoretical weight: 16.15 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q8RWA7 (Residues: 1-143; Coverage: 100%)
Gene names: At1g04630, MEE4, T1G11.12
Pfam: GRIM-19 protein
InterPro: GRIM-19

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Chain: q
Length: 159 amino acids
Theoretical weight: 18.35 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9M9M9 (Residues: 1-159; Coverage: 100%)
Gene names: At3g03100, T17B22.21
Pfam: NADH ubiquinone oxidoreductase subunit NDUFA12
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

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Chain: r
Length: 131 amino acids
Theoretical weight: 15.06 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9SD78 (Residues: 1-131; Coverage: 100%)
Gene names: At5g08060, At5g08060/F13G24_260, F13G24.260, T22D6.4

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