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Structural basis for bacterial lipoprotein relocation by the transporter LolCDE.

Tang X, Chang S, Zhang K, Luo Q, Zhang Z, Wang T, Qiao W, Wang C, Shen C, Zhang Z, Zhu X, Wei X, Dong C, Zhang X, Dong H
Nat Struct Mol Biol 28 347-355 (2021)
Related entries: 7ari, 7arj, 7ark, 7arl, 7arm

Cited: 18 times
EuropePMC logo PMID: 33782615

Abstract

Lipoproteins in the outer membrane of Gram-negative bacteria are involved in various vital physiological activities, including multidrug resistance. Synthesized in the cytoplasm and matured in the inner membrane, lipoproteins must be transported to the outer membrane through the Lol pathway mediated by the ATP-binding cassette transporter LolCDE in the inner membrane via an unknown mechanism. Here, we report cryo-EM structures of Escherichia coli LolCDE in apo, lipoprotein-bound, LolA-bound, ADP-bound and AMP-PNP-bound states at a resolution of 3.2-3.8 Å, covering the complete lipoprotein transport cycle. Mutagenesis and in vivo viability assays verify features of the structures and reveal functional residues and structural characteristics of LolCDE. The results provide insights into the mechanisms of sorting and transport of outer-membrane lipoproteins and may guide the development of novel therapies against multidrug-resistant Gram-negative bacteria.

Articles - 7arh mentioned but not cited (1)

  1. Cryo-EM structures of LolCDE reveal the molecular mechanism of bacterial lipoprotein sorting in Escherichia coli. Bei W, Luo Q, Shi H, Zhou H, Zhou M, Zhang X, Huang Y. PLoS Biol 20 e3001823 (2022)


Reviews citing this publication (3)

  1. ABC Transporters in Bacterial Nanomachineries. Bilsing FL, Anlauf MT, Hachani E, Khosa S, Schmitt L. Int J Mol Sci 24 6227 (2023)
  2. Bacterial Lipoprotein Posttranslational Modifications. New Insights and Opportunities for Antibiotic and Vaccine Development. Smithers L, Olatunji S, Caffrey M. Front Microbiol 12 788445 (2021)
  3. Structure and mechanism of the bacterial lipid ABC transporter, MlaFEDB. Ekiert DC, Coudray N, Bhabha G. Curr Opin Struct Biol 76 102429 (2022)

Articles citing this publication (14)

  1. Conformational snapshots of the bacitracin sensing and resistance transporter BceAB. George NL, Schilmiller AL, Orlando BJ. Proc Natl Acad Sci U S A 119 e2123268119 (2022)
  2. Disorder is a critical component of lipoprotein sorting in Gram-negative bacteria. El Rayes J, Szewczyk J, Deghelt M, Csoma N, Matagne A, Iorga BI, Cho SH, Collet JF. Nat Chem Biol 17 1093-1100 (2021)
  3. Mechanism of LolCDE as a molecular extruder of bacterial triacylated lipoproteins. Sharma S, Zhou R, Wan L, Feng S, Song K, Xu C, Li Y, Liao M. Nat Commun 12 4687 (2021)
  4. Structural basis for heme detoxification by an ATP-binding cassette-type efflux pump in gram-positive pathogenic bacteria. Nakamura H, Hisano T, Rahman MM, Tosha T, Shirouzu M, Shiro Y. Proc Natl Acad Sci U S A 119 e2123385119 (2022)
  5. A comparative analysis of lipoprotein transport proteins: LolA and LolB from Vibrio cholerae and LolA from Porphyromonas gingivalis. Jaiman D, Nagampalli R, Persson K. Sci Rep 13 6605 (2023)
  6. ATP-Binding Cassette Transporters: Snap-on Complexes? Younus I, Kochkina S, Choi CC, Sun W, Ford RC. Subcell Biochem 99 35-82 (2022)
  7. Deep learning-guided discovery of an antibiotic targeting Acinetobacter baumannii. Liu G, Catacutan DB, Rathod K, Swanson K, Jin W, Mohammed JC, Chiappino-Pepe A, Syed SA, Fragis M, Rachwalski K, Magolan J, Surette MG, Coombes BK, Jaakkola T, Barzilay R, Collins JJ, Stokes JM. Nat Chem Biol (2023)
  8. Feeling at home: Structure of the NTSR1-Gi complex in a lipid environment. Maharana J, Shukla AK. Nat Struct Mol Biol 28 331-333 (2021)
  9. Identification of an Essential LolD-Like Protein in Helicobacter pylori. McClain MS, Bryant KN, McDonald WH, Algood HMS, Cover TL. J Bacteriol 205 e0005223 (2023)
  10. Mechanistic insights into the regulation of cell wall hydrolysis by FtsEX and EnvC at the bacterial division site. Xu X, Li J, Chua WZ, Pages MA, Shi J, Hermoso JA, Bernhardt T, Sham LT, Luo M. Proc Natl Acad Sci U S A 120 e2301897120 (2023)
  11. Regulation of the cell division hydrolase RipC by the FtsEX system in Mycobacterium tuberculosis. Li J, Xu X, Shi J, Hermoso JA, Sham LT, Luo M. Nat Commun 14 7999 (2023)
  12. Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410. Remm S, De Vecchis D, Schöppe J, Hutter CAJ, Gonda I, Hohl M, Newstead S, Schäfer LV, Seeger MA. Nat Commun 14 6449 (2023)
  13. Structural basis of lipoprotein recognition by the bacterial Lol trafficking chaperone LolA. Kaplan E, Greene NP, Jepson AE, Koronakis V. Proc Natl Acad Sci U S A 119 e2208662119 (2022)
  14. Structures of the CcmABCD heme release complex at multiple states. Li J, Zheng W, Gu M, Han L, Luo Y, Yu K, Sun M, Zong Y, Ma X, Liu B, Lowder EP, Mendez DL, Kranz RG, Zhang K, Zhu J. Nat Commun 13 6422 (2022)


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